汉防己甲素对增生性瘢痕基质胶原酶活性的影响  

The effects of tetrandrane on activity of collagenase derived from human hypertrophic scar

作　　者：曹治东[1] 石崇荣[2] 黄崇本[2] 李邦春[1] 苟景跃[1] 刘远禄[1] 张奎[1] 

机构地区：[1]重庆市第四人民医院(重庆市急救医疗中心)骨科,400014 [2]重庆医科大学附属第一医院整形外科

出　　处：《中华整形外科杂志》2006年第6期448-450,共3页Chinese Journal of Plastic Surgery

摘　　要：目的观察汉防己甲素对瘢痕胶原酶活性的影响,进一步阐明其抗瘢痕作用的机理。方法将汉防己甲素的实验浓度控制在低于细胞增殖抑制范围之内,采用加胶原蛋白底物的SDS-PAGE电泳法分离胶原酶,胰蛋白酶激活后利用湿胶密度扫描仪分析胶原酶的活性。同时采用改良的氯胺T法测量细胞外胶原蛋白量,并分析两者的相关关系。结果在不抑制细胞增殖的情况下,汉防己甲素可显著提高胶原酶的活性,使细胞外胶原蛋白减少,两者呈显著的负相关,此作用强于激素。结论增强胶原酶降解胶原蛋白的活性,减少细胞外胶原沉积是汉防己甲素抗瘢痕作用机理之一。Objective To observe the effect of tetrandrane on activity of collagenase derived from human hypertrophic scar for the sake of clarifying the mechanism as tetrandrane acting on scar. Methods The experimental concentration was controled below that of cellproliferation inhibited, SDS-PAGE electrophoresis was adopted to separate collagenase from extracellular matrix, and then activated by trypsin analyzed the activity of collagenase with density scaning apparatus. At the same time quantity of extracellular collagen was measured using improved chloraseptine T oxidizing assay, moreover analyzed correlation between activity of collagenase and quantity of extracellular collagen. Results In the concentration below the lever of inhibiting fibroblast proliferation, the total activity of collagenase could be significantly increased by tetrandrane with dosage- dependence associated with quantity of extracellular collagen reduced, which was much greater than that of triamcinolone. Conclusion Increasing activity of collagenase on degradation of collagen even in a lower concentration was one of the mechanisms of tetrandrane treating hypertrophic scar.

关 键 词：汉防己甲素 增生性瘢痕 胶原酶 

分 类 号：R285.5[医药卫生—中药学]