阴道念珠菌病小鼠模型中单核细胞趋化蛋白-1的表达及其意义  

Expression of MCP-1 in Mice with Experimental Vaginal Candidiasis and Potential Significance

作　　者：严小枫[1] 李晖 林能兴[1] 陈宏翔[1] 黄长征[1] 刘志香[1] 邬炎卿[1] 张德美[1] 涂亚庭[1] 

机构地区：[1]华中科技大学同济医学院附属协和医院皮肤科,湖北武汉430022 [2]宜昌市第二人民医院皮肤科,湖北宜昌443000

出　　处：《中国皮肤性病学杂志》2006年第11期662-665,共4页The Chinese Journal of Dermatovenereology

摘　　要：目的检测阴道念珠菌病小鼠模型中单核细胞趋化蛋白-1(MCP-1)的表达,并对其在阴道念珠菌病发病中的作用进行探讨。方法建立阴道念珠菌病小鼠模型,以免疫组化染色SABC法检测小鼠阴道黏膜中MCP-1蛋白的表达,并应用HMIAS-2000型全自动医学彩色图像分析系统对小鼠阴道黏膜MCP-1的表达进行半定量分析。结果与对照组及正常小鼠相比,感染组小鼠阴道黏膜MCP-1表达水平明显升高,且随时间延长逐渐升高,14天达到高峰,第21天略有下降。结论MCP-1可能在局部阴道黏膜内对白念珠菌的感染起一定的防御作用。Objective To investigate the role of MCP-1 during a Candida albicans vaginal infection by detecting its expression in mice with experimental vaginal candidiasis. Methods To set up a murine model of vaginal candidiasis, and to detect the expression of MCP-1 in vaginal tissue by immunohistochemistry. Semi-quantitative measurement of MCP-1 expression in vaginal tissue was done with High Multiply Image Analytical System （HMIAS）. Results The expression of MCP-1 in vaginal tissue of infected mice was significantly higher than those in controls and normal mice. As time going on, the expression of MCP-1 increased, and reached its climax at day 14 ,than declined a little on day 21. Conclusion MCP-1 may play a role in the local vaginal mucosal response to C. albicans.

关 键 词：念珠菌病 外阴阴道 白念珠菌 单核细胞趋化蛋白质1 

分 类 号：R711.31[医药卫生—妇产科学]