葛根素对糖尿病肾病大鼠细胞间黏附分子-1基因表达的调控  

Effect of puerarin on ICAM-1 gene expression in streptozotocin-induced diabetic nephropathy rats

作　　者：李强翔[1] 钟惠菊[1] 李剑欣[2] 贺金莲[2] 张卓[3] 陆小兰[1] 

机构地区：[1]中南大学湘雅医院内分泌科,湖南长沙410008 [2]湖南省娄底市中心医院药剂科 [3]湖南省娄底市中心医院内分泌科

出　　处：《中国医师杂志》2006年第11期1480-1483,共4页Journal of Chinese Physician

基　　金：湖南省衡阳市科委课题资助项目(2005ks01-43)

摘　　要：目的观察葛根素对糖尿病大鼠肾小球细胞间黏附分子ICAM-1基因表达的调控。方法利用链脲佐菌素腹腔注射法诱导建立1型糖尿病大鼠模型,将实验用SD大鼠随机分为正常对照组、糖尿病组、葛根素治疗组。治疗16周。观察治疗期间及治疗后大鼠的一般状况、血糖、尿素氮、血肌苷,内生肌苷清除率、24 h尿蛋白排泄率,原位杂交检测肾组织CD54基因表达。结果造模组大鼠均出现肾脏功能有损害;葛根素能改善基本状况,降低糖尿病大鼠的尿素氮、血肌苷、24 h尿白蛋白排泄率,增加内生肌苷清除率,细胞间黏附分子mRNA表达显著下调。结论葛根素具有确切的肾脏保护作用。Objective To observe the effect of puerarin on the ICAM-1 gene expression in streptozotocin-lnduced diabetic nephropathy rats. Methods Diabetic nephropathy rats were induced by intraperltoneal injection of STZ. Rats were allocated into normal control group, model group, and puerarin group. The puerarin treatment lasted for 16 weeks. During and after the treatment , the general health conditions, blood glucose levels , blood urea nitrogen , serum creatinine, urinary albumin excretion rate of 24-hours, and clearance rate of creatinine of the rats were observed. The ICAM-1 mRNA expressions were also determined by in situ hybridization analysis in the kidney tissue of the rats. Results Diabetes mellitus and renal function lesion occurred in the model group and puerarin group. Puerarin could improve the general health of the rats, decrease blood urea nitrogen , serum creatinine, and urinary albumin excretion rate of 24-hours ; and increase the clearance rate of creatinine. The ICAM-1 mRNA expressions in the kidney were significantly inhibited by puerarin treatment. Conclusion Puerarin has a function of renal protection.

关 键 词：葛根素/药理学 糖尿病肾病 胞间粘附分子1 

分 类 号：R285[医药卫生—中药学]