骨髓基质细胞对背根神经节细胞生长的影响  

Effect of bone marrow stromal cells on the growth of dorsal root ganglia cells

作　　者：王洁[1] 周欣阳[1] 刘炎[1] 丁斐[1] 顾晓松[1] 

机构地区：[1]南通大学江苏省神经再生重点实验室,南通226001

出　　处：《南通大学学报（医学版）》2006年第6期399-402,共4页Journal of Nantong University(Medical sciences)

基　　金：国家自然科学基金资助项目(30540063);江苏省自然科学基金资助项目(BK2005202);江苏省高校自然科学基金(04KJB180112)

摘　　要：目的:体外研究骨髓基质细胞(Bonemarrowstromalcells,MSCs)对背根神经节细胞生长的影响。方法:采用差速贴壁的方法分离、培养大鼠骨髓基质细胞。经transwell膜与新生大鼠背根神经节细胞联合培养。利用MTT、细胞免疫荧光组织化学方法检测骨髓基质细胞对背根神经节细胞生长的影响。结果:联合培养后的新生大鼠背根神经节细胞活力增加,神经突起增长。结论:骨髓基质细胞对于背根神经节细胞的活力以及神经突起生长有一定的促进作用。Objectives： To investigate the effect of bone marrow stromal cells on cultured dorsal root ganglia cells（DRGs）. Methods： Rat MSCs were isolated and cultured from bone marrow cells by their strong adherence. Using transwell inserts, we established an indirect co-culture system with DRGs, MTT assay and immunocytochemical analysis were conducted to detect the influence of MSCs on DRGs. Results： The cell vitality of co-cultured DRGs was increased, and their neural growth was also improved. Conclusion： Bone marrow stromal cells play an important role in promoting the vitality and growth of dorsal root ganglia cells.

关 键 词：骨髓基质细胞 背根神经节细胞 联合培养 大鼠 

分 类 号：R318[医药卫生—生物医学工程]