羧基化碳纳米管修饰的安培型葡萄糖传感器测定血液中葡萄糖  

Determination of Glucose in Serum by Amperometric Glucose Biosensor Modified with Carboxylic Carbon Nanotubes

作　　者：于巧玲[1] 杜亚琳[2] 孙晓艳[2] 

机构地区：[1]桂林工学院资源与环境工程系,广西桂林541004 [2]桂林工学院材料与化学工程系,广西桂林541004

出　　处：《分析测试学报》2006年第6期31-34,共4页Journal of Instrumental Analysis

基　　金：广西教育厅科研基金资助课题(桂教科研[2005]47号)

摘　　要：羧基化碳纳米管修饰的葡萄糖传感器是以二茂铁为电子媒介体，利用CNTs上的羧基与GOx的氮基和羧基相互作用固定酶，表观米氏常数Km=1．77×10mol／L。考察了温度、酸度、富集时间、扫速对测定的影响，酶电极的检出限为1×10^-8mol／L（S／N=3），线性范围为1．5×10^-7-4×10^-5mol／L。尿酸对测定无干扰，用于人血清中葡萄糖含量的测定，回收率在98％-105％之间。The glucose biosensor was modified with carboxylic carbon nanotube. By using ferrocene as an efficient electron transfer mediator between the glucose oxidase and the electrode in the modified glucose biosensor, the stability of the electrode and the sensitivity of determination could be enhanced. Glucose oxidase（GOx） could be bound on the modified electrode through the interaction of the carboxyl group in the carbon nanotubes with the amino and carboxyl groups in glucose oxidase. The apparent Michaelis- Menten constant, Km was 1. 77 × 10^-44 mol/L. The effects of temperature, acidity, enrichment time, and the scan rate on the peak current were investigated. The linear range was 1.5 × 10^-7 - 4 × l0^-5 mol/L with a detection limit of 1 × 10^-8 mol/L（S/N=3）. Uric acid did not interfere with the determination. The biosensor has been applied to determine human serum glucose with recoveries ranging from 98% to 105%.

关 键 词：碳纳米管 二茂铁 葡萄糖氧化酶 葡萄糖 

分 类 号：O657.15[理学—分析化学] Q532.3[理学—化学]