2,3,4,9-四氢-9-羟基-1,10-蒽醌的合成及荧光猝灭法测定痕量砷  

Fluorescence Quenching Method for the Determination of Arsenic with H_2O_2-2,3,4,9-Tetrahydrogen-9-Hydroxy-1,10-Anthraquinone System

作　　者：胡世荣[1] 杨天隆[1] 刘琴英[1] 周丽红[1] 

机构地区：[1]漳州师范学院化学系,福建漳州363000

出　　处：《分析测试学报》2006年第6期60-62,66,共4页Journal of Instrumental Analysis

基　　金：福建省教育基金资助项目(JA04245;JA04277)

摘　　要：基于HCl介质中和α，α′-联吡啶存在下，As（V）可催化H2O2氧化2，3，4，9-四氢-9-羟基-1，10-蒽醌（R）转化为无荧光化合物，导致体系荧光急剧猝灭，建立了催化H2O2氧化R荧光猝灭法测定痕量砷的新方法。在最佳条件下，As（V）含量在0．0040～0．40μs／L范围内与荧光强度的△If值呈线性关系，工作曲线的回归方程△If=11．94＋233．7 ρAs（V）（μg／L），n=7，相关系数r=0．9994，As（V）检出限为0．62ng/L，n=11。对0．0040和0．40μg／LAs（Ⅴ）分别进行11次的测定，其RSD为2．6％与3．8％。该方法重复性好、灵敏、准确，用于实际样品中砷含量的测定，结果满意。还报道了R的合成，并借助核磁共振潜、红外光谱及质谱等谱学方法确定了其结构。A novel fluorescence method was developed for the determination of arsenic with H2O2 -2, 3,4,9-tetrahydrogen-9-hydroxy-1,10-anthraquinone system. It is based on the catalyzing effect of As （ Ⅴ ） on the fluorescence quenching of 2,3,4,9-tetrahydrogen-9-hydroxy-1,10-anthraquinone （R） by H2O2 in the presence of α,α′-dipyridine in HCl solution. Under optimum conditions, the linear range for As（Ⅴ） was 0. 004 0 -0. 40 μg/L with a correlation coefficient of 0. 999 4 and a detection limit of 0. 62 ng/L. The relative standard deviations for 0. 004 0 μg/L and 0. 40 μg/L As （Ⅴ ） were 2.6% and 3.8% , respectively. The method has been successfully applied to the determination of trace As （ Ⅴ ） in real samples with satisfactory results. The synthesis of R was also reported.

关 键 词：砷 荧光猝灭法 α α′-联吡啶 蒽醌 

分 类 号：O657.15[理学—分析化学] Q425[理学—化学]