流动注射化学发光法测定盐酸仑氨西林  

Determination of Lenampicillin Hydrochloride by Flow Injection Chemiluminescence method

作　　者：何树华[1] 

机构地区：[1]涪陵师范学院化学系,重庆408005

出　　处：《分析测试学报》2006年第6期115-116,119,共3页Journal of Instrumental Analysis

基　　金：重庆市教委科学研究项目(KJ051305)

摘　　要：基于鲁米诺在碱性条件下可以被过氧化氢氧化产生化学发光，盐酸仑氨西林对该发光有显著增强作用这一现象，结合流动注射技术，建立了一种直接测定盐酸仑氨西林的化学发光新方法。该方法具有较高的灵敏度，检出限为5．6μg·L^-1（3σ），线性范围为0．01—10mg·L^-1，对0．1mg·L^-1盐酸仑氨西林平行测定11次，其相对标准偏差为3．6％。该方法已成功用于片剂和血清中盐酸仑氨西林的测定。A novel chemiluminescence method coupled with flow injection technique for the determination of lenampicillin hydrochloride is developed. It is based on the enhancement of lenampicillin hydrochloride on the chemiluminescence reaction of luminol with H2O2 in sodium hydroxide medium. The chemiluminescence intensity is linear with the concentration of lenampicillin hydrochloride in the range of 0. 01 - 10mg·L^-1 The RSD for 0. 1 mg·L^-1lenampicillin hydrochloride is 3.6% （n = 11 ）, and the detection limit is 5.6 μg·L^-1. This method has been successfully applied to the determination of lenampicillin hydrochlofide in tablets and serum.

关 键 词：化学发光 鲁米诺-过氧化氢 流动注射分析 盐酸仑氨西林 

分 类 号：O657.31[理学—分析化学] O613.63[理学—化学]