高效液相色谱-脉冲积分安培电化学检测牛奶中的妥布霉素  

Determination of Tobramycin in Milk by HPLC with Pulsed Integrated Amperometric Electrochemical Detection

作　　者：蔡玉娥[1] 蔡亚岐[1] 牟世芬[1] 鲁毅强[2] 

机构地区：[1]中国科学院生态环境研究中心环境化学与生态毒理学国家重点实验室,北京100085 [2]北京科技大学化学系,北京100083

出　　处：《分析测试学报》2006年第6期117-119,共3页Journal of Instrumental Analysis

基　　金：国家重点基础研究计划(973项目)(2003CB415001);国家自然科学基金资助项目(20577058;20475060);中国科学院优秀博士论文基金资助课题

摘　　要：采用C18键合硅胶柱和脉冲积分安培电化学检测器对妥布霉素样品进行了分离检测。对两种检测电位波形进行了对比，并选取了表现优良的检测电位。方法对妥布霉素的检出限19μg／L，在0．1～25mg／L范围内表现良好线性（r=0．9999）。该方法应用于市售鲜奶的检测，妥布霉素的回收率为98％。A new method was developed for the separation and determination of tobramycin in milk by HPLC using a C18 column and pulsed integrated amperometric electrochemical detection. Two detection potential waveforms were compared and the one that gave the least noise level was selected. The linear range of the calibration curve for tobramycin was 0. 1 - 25 mg/L with a correlation coefficient of 0. 999 6. The detection limit was 19 μg/L and the relative standard deviation was 1.8% （n = 10）. The spiked recovery was 98%. The method has been applied to the determination of tobramycin in milk sample.

关 键 词：妥布霉素 液相色谱 脉冲积分安培检测 

分 类 号：O657.72[理学—分析化学]