黄芩中黄芩苷的抗内毒素作用  

Anti-endotoxin Effects of Baicalin Extracted from Radix Scutellariae

作　　者：李敬[1] 刘云海[1] 陈新[2] 丁小佳[1] 伍三兰[3] 谢委[1] 

机构地区：[1]华中科技大学同济医学院附属同济医院药学部,武汉430030 [2]武汉工业学院药剂教研室,430023 [3]华中科技大学同济医学院药学院,武汉430030

出　　处：《医药导报》2006年第12期1237-1240,共4页Herald of Medicine

基　　金：国家自然科学基金资助项目(基金编号:39870872);卫生部科学研究基金资助项目(基金编号:98-2-110)

摘　　要：目的探讨黄芩中黄芩苷的抗内毒素作用。方法将黄芩苷配成0.5%水溶液,鲎实验法进行抗内毒素定量测定;内毒素致家兔发热实验检测黄芩苷体内抗内毒素作用;脂多糖致小鼠死亡实验测定黄芩苷保护作用及黄芩苷对脂多糖致小鼠过度释放肿瘤坏死因子(TNF)和一氧化氮(NO)的影响实验,研究黄芩苷的抗内毒素作用。结果0.833 g.mL-1黄芩苷可使4 EU内毒素降解为0.546 EU,破坏率为87.45%;0.5%黄芩苷溶液可显著降低内毒素引起的家兔体温升高,同等剂量脂多糖引起的小鼠死亡率从70.0%降为10.0%;黄芩中的黄芩苷可抑制脂多糖致小鼠血清中TNF和NO的过度释放,其抑制率呈剂量依赖性。结论从黄芩中提取分离出的黄芩苷有抗内毒素作用。Objective To probe into the anti-endotoxin effects of baicalin isolated from Radix Scutellariae. Methods A 0.5% aqueous solution of baicalin was prepared which was extracted and isolated from Radix Scutellariae, The in vitro antiendotoxin effect of baicalin was quantitatively determined with the limulus test after a mixture of endotoxin and baicalin had been incubated in a 37 ~C water bath for 1 h. The in vitro inhibitory of baicalin on the endotoxin-induced fever was measured in rabbits. The LPS-induced deaths in mice pretreated and not pretreated with baicalin were compared. Effects of baicalin on the LPS induced excessive increase in the serum contents of tumor necrosis factor-c^（TNF-ct） and nitric oxide （NO） were studied in mice. Results 0.833 mg ~ mL＂~ of baiealin was shown to result in a degradation of endotoxin from 4 EU to 0. 546 EU, the destruction percentage being 87.45%. 5 mL·kg^-1 of 0.5% baicalin solution injected intravenously per rabbit gave rise to a striking abatemant of the endotoxin-induced fever. The LPS-induced death rate in mice pretreated with baicalin （ 10% ） was significantly lower than that in those without baicalin pretreatment（70% ）, The excessive increase in the serum TNF-α and NO induced by LPS in mice was brought down significantly by baicalin in a dose-dependent manner. Conclusion Baicalin isolated from Radix Scutellariae was shown to be provided with anti-endotoxin effects.

关 键 词：黄芩 黄芩苷 内毒素 脂多糖 肿瘤坏死因子 一氧化氮 

分 类 号：R282.71[医药卫生—中药学] R965[医药卫生—中医学]