多通道SPR影像传感器及其对DNA的特异性检测  

Multi-Channel SPR Imaging Sensor and Specific Detection of DNA

作　　者：刘国华[1] 张福海[1] 俞梅[1] 贾芸芳[1] 张维[1] 岳钊[1] 常露[1] 牛文成[1] 

机构地区：[1]南开大学信息技术科学学院,天津300071

出　　处：《电子学报》2006年第11期1990-1993,共4页Acta Electronica Sinica

基　　金：国家自然科学基金(No.60574091);天津市科技攻关项目(No.06YFGPGX08700)

摘　　要：SPR传感器的响应包括多种成份,除了待测分子与探针分子相互结合引起的响应(特异性响应)外,还有样液中其它成份及其浓度的变化、温度变化以及非待测分子与敏感膜的相互作用引起的响应(非特异性响应).后者的存在会严重影响SPR传感器的测量精度.本文提供了一种提高检测精度的方法,即采用多通道SPR影像传感器结构,通过不同通道之间的比较,提取出待测分子与探针分子之间相互作用所引起的响应,在真正意义上实现了对生物分子相互作用的实时、动态检测.通过对DNA的检测,证明这种方法对生物分子相互作用的特异性检测是行之有效的.The response of SPR sensor includes two components： specific response and non-specific response. The former is due to the capture of analyte molecules by target molecules immobilized on the sensor surface, and the latter is due to the adsorption of non-target molecules to ,sensor surface and temperature fluctuations or sample variations of composition and concentration. The existence of non-specific response reduces the precision of SPR sensors. In this paper we provide a kind of multi-channel SPR imaghag sensor structure to improve the accuracy of SPR sensors. It can discriminate between the specific response and the non-specific response by comparison of different channels, and realize the real-time detection of the biomolecular interactions, This method is demonstrated effective on specific detection by testing for DNA.

关 键 词：表面等离子共振 多通道影像传感器 特异性响应 DNA 

分 类 号：TP212.3[自动化与计算机技术—检测技术与自动化装置]