PKC抑制剂Ro31－8220对大鼠肝产生IL－6和纤维蛋白原的作用  

Effect of PKC inhibitor Ro31-8220 on the production of interleukin-6 and fibrinogen in rat liver

作　　者：林文[1] 冯增辉[1] 张俊平[1] 胡振林[1] 钱定华[1] 

机构地区：[1]第二军医大学药学院中西药研究室

出　　处：《第二军医大学学报》1996年第6期528-530,共3页Academic Journal of Second Military Medical University

基　　金：国家自然科学基金

摘　　要：目的：研究重组人白细胞介素６（ｒｈＩＬ－６）对原代培养大鼠肝细胞分泌纤维蛋白原（Ｆｉｂｒｉｎｏｇｅｎ，Ｆｇｎ）的影响及蛋白激酶Ｃ（ＰＫＣ）抑制剂Ｒｏ３１－８２２０对枯否细胞分泌ＩＬ－６的作用。方法：以酶联免疫吸附测定法（ＥＬＩＳＡ）测定Ｆｇｎ；测定Ｉ－６采用［３Ｈ］－ＴｄＲ掺入增殖法。结果：大鼠肝细胞（３×１Ｏ６／ｍｌ）与ｒｈＩＬ－６（０～１００ｋＵ／Ｌ）孵育２４ｈ，刺激肝细胞分泌Ｆｇｎ增加；Ｒｏ３１－８２２０（０．１～１０μｍｏｌ／Ｌ）显著抑制枯否细胞释放ＩＬ－６。结论：提示ＩＬ－６是调节纤维蛋白原合成的重要细胞因子，推测ＰＫＣ抑制剂可通过抑制枯否细胞分泌ＩＬ－６来降低Ｆｇｎ的分泌。To investigate the effect of interleukin-6 (IL-6) on the fibrinogen production from rat primary hepatocytes culture and Ro31-8220 on the IL-6 production from Kupffer cells. Methods: Enzyme link immunosorbent assay(ELISA) was used to determine fibrinogen levels in hepatocyte culture and IL-6 level was determined by [3H]-TdR incorporation assay. Results: IL-6 (0～100 kU/L) dose-dependently enhanced fibrinogen production after cultured with hepatocytes(3×106/ml) for 24 h and Ro31-8220 (0.1～10 umol/L) markedly reduced IL-6 secretion. Conclusion: IL-6 plays a crucial role in modulation of fibrinogen and PKC inhibitors could decrease fibrinogen production through the inhibition of IL-6 secretion from K upffer cells.

关 键 词：白细胞介素6 纤维蛋白原 肝细胞 蛋白激酶C 

分 类 号：R977.3[医药卫生—药品] R967[医药卫生—药学]