水和生物体液中曲马多镇痛药的中空纤维膜液相微萃取  

Hollow Fiber Liquid Phase Microextraction of Tramadol from Water and Biological Samples

作　　者：王晓飞[1] 刘俊亭[1] 王妍[1] 赵秀丽[1] 高丽娜[1] 徐冬辉[1] 

机构地区：[1]中国医科大学药学院,辽宁沈阳110001

出　　处：《色谱》2006年第6期641-644,共4页Chinese Journal of Chromatography

基　　金：国家自然科学基金资助项目(No.30271447)

摘　　要：使用中空纤维膜液相微萃取技术(LPME-HFM)建立了从水和生物样品(尿和血浆)中提取曲马多的方法。在室温(20℃)下用聚偏氟乙烯中空纤维膜过滤提取样品。萃取过程中用4μL甲苯作为萃取溶剂。用度冷丁作为内标,气相色谱法-氢火焰离子化检测器分析测定,最低检测限达0.01 mg/L(自来水、尿)或0.05 mg/L(血浆)。和传统的液液萃取方法相比,该方法集萃取和浓缩一步完成,更简便、快速、绿色环保。A novel technique of liquid phase microextraction supported by hollow fiber membrane （LPME-HFM） was applied to extract tramadol in tap water, urine and human plasma. At room temperature （20℃ ）, polyvinylidene fluoride hollow fiber membrane （ PVDF-HFM ） was employed. In the procedure of extraction, 4 μL of organic solvent （toluene） was used in individual extraction. Pethidine was used as the internal standard （IS） in the sample analysis by gas chromatography with flame ionization detector （GC-FID）. The optimized conditions for tramadol extraction was as follows： toluene as organic solvent, 4 mL sample solution added with 0. 1 mL of 1 mol/L NaOH solution, extraction time of 15 min, and stirring speed of 300 r/min. The sample solutions, containing a series of concentrations of tramadol, 0.05, 0. 10, 0.50, 1.0, 5.0, 10.0 mg/L, were analyzed. The good linearities were obtained for water, human urine and human plasma samples with the correlation coefficients of 0. 999 7, 0. 994 8 and 0. 998 5, respectively. The detection limits can reach 0.01 mg/L for water and human urine and 0.05 mg/L for human plasma. The relative standard deviations （RSDs） were 10. 6% and 5. 15% for water, 18. 1% and 4.03% for urine solution at the spiked levels of 0. 1 and 1.0 mg/L. The RSDs were 16.6% and 4. 15% for plasma solution at the spiked levels of 0.5 and 1.0 mg/L. In comparison with conventional method, this method is simple, sensitive, fast, inexpensive, and environmentally friendly. It can be applied to pharmaceutical and forensic analysis.

关 键 词：液相微萃取 中窀纤维膜 气相色谱 曲马多 度冷丁 

分 类 号：O658[理学—分析化学]