磷酸苯丙哌林对映体的毛细管电泳手性分离及应用  

Enantioseparation of Benproperine Phosphate by Capillary Electrophoresis and Its Application

作　　者：何敏[1] 赵书林[1] 白文玲[1] 王兵[1] 

机构地区：[1]广西师范大学化学化工学院,桂林541004

出　　处：《分析化学》2006年第U09期59-62,共4页Chinese Journal of Analytical Chemistry

基　　金：国家自然科学基金(No.20265001);广西教育厅科学基金资助项目

摘　　要：以羟丙基-γ-环糊精（HP-γ-CD）为手性选择剂，采用毛细管电泳技术，对手性药物磷酸苯丙哌林的对映体拆分进行了研究。在以含有10mmol／L HP-γ-CD 100mmol／L Tris-H3PO4（pH3．0）缓冲溶液为电泳电解质时，磷酸苯丙哌林对映体在25min内实现了基线分离，分离度达2.03。以紫外检测（测定波长202nm）时，其峰面积与磷酸苯丙哌林对映体的浓度在0．5～10mg／L范围内呈良好的线性关系。将其应用于合成的血清样品分析，获得了满意的结果。实验还尝试采用场放大堆积方法，对磷酸苯丙哌林对映体进行在柱富集，使磷酸苯丙哌林对映体的检测灵敏度提高了23倍。A new method for the chiral separation of benproperine phosphate enantiomers is reported. The enantioseparation of benproperine phosphate was obtained by capillary electrophoresis using hydroxyproply-γ-cyclodextrin（HP-γ-CD） as chiral selector. When 100 mmol/L Tris-H3 PO4 buffer at pH 3.0 containing 25 mmol/L HP-γ-CD was chosen as a running electrolyte, the benproperine phosphate enantiomers was resolved completely within 25 min and the resolution reached up to 2.03. Coupled with ultraviolet detection, an excellent linear range from 0.5 mg/L to 10 mg/L for the detemination of benproperine phosphate enantiomers was exhibited. The enantioseparation method has been applied for the determination of benproperine phosphate enantiomer spiked in serum samples with satisfactory results. A field-amplified injection procedure was also investigated as a simple and effective way to enhance the sensitivity for the determination, this procedure can improve the detection sensitivity of benproperine phosphate enantiomers up to 23 folds.

关 键 词：毛细管电泳 对映体分离 磷酸苯丙哌林 

分 类 号：O658.9[理学—分析化学]