用L-脯氨酸衍生物作为手性流动相添加剂拆分对映异构体  

Separation of Enantiomers by High Performance Liquid Chromatography Using Derivants of L-Proline as Chiral Mobile Phase Additives

作　　者：常银霞[1] 候志林[1] 黎其万[2] 高天荣[1] 袁黎明[1] 

机构地区：[1]云南师范大学化学化工学院 [2]云南省农业科学院质量标准与检测技术研究所,昆明650223

出　　处：《分析化学》2006年第U09期100-104,共5页Chinese Journal of Analytical Chemistry

基　　金：国家自然科学基金(No.30160092);高等学校优秀青年教师教学科研奖励计划;云南省自然科学基金(No.2004B0013R)资助项目

摘　　要：用自制的N-十二酰基-L-脯氨酸和N-十二酰基-L-脯氨酸-3,5-二甲基苯胺两种L-脯氨酸衍生物作手性流动相添加剂,在氨丙基硅烷化硅胶柱上,用正己烷/异丙醇作流动相,对多种手性化合物进行了高效液相色谱拆分。实验结果表明:用N-十二酰基-L-脯氨酸作添加剂拆分的12种手性化合物,有8种手性化合物能得到拆分,具有较好的手性选择性。虽然用N-十二酰基-L-脯氨酸-3,5-二甲基苯胺作添加剂,手性选择性也较好,但由于含有苯环,紫外吸收增强,基线波动严重。N-dodecanoyl-L-proline and N-dodecanoyl-L-proline-3,5-dimethylanilide derived from L-proline were used as chiral mobile phase additives. Enantiomers were separated using hexane and isopropanol as mobile phase on a aminopropyl silica gel column by high performance liquid chromatography. The results show that the enantioselectivity of N-dodecanoyl-L-proline is better and eight enantiomers of the twelve enantiomers can be separated. The enantioselectivity of N-dodecanoyl-L-proline-3,5-dimethyl-anilide is also good, but the basic line fluctuates strongly due to containing benzene ring in its molecular structure.

关 键 词：N-十二酰基-L-脯氨酸 N-十二酰基-L-脯氨酸-3 5-二甲基苯胺 高效液相色谱 流动相添加剂 

分 类 号：R917[医药卫生—药物分析学]