快原子轰击质谱的非线性拟合法定量分析取代基海因  

Analysis of Substituted Hyantoin by Fast Atom Bombardment Mass Spectrometry/Non-linear Curve Fitting Method

作　　者：郭志峰[1] 李国俊[1] 

机构地区：[1]河北大学理化分析研究中心

出　　处：《分析化学》2006年第U09期138-140,共3页Chinese Journal of Analytical Chemistry

摘　　要：采用快原子轰击质谱法，在有底物甘油的条件下，测定f-5，5-二甲基海因，5-甲基-5-乙基海因的准分子离子峰[M＋H]^＋的质量色谱图，通过对其曲线拟合，发现它们的强度随采集时间呈指数衰减规律，并且，二者初始峰强的比值与仅与二者的浓度有关。当选定一个作为内标，则可对另一个定量分析。实验选择5-甲基-5-乙基海因作为内标，对5，5-二甲基海因定量分析。测得标准曲线方程为y=-0．0204＋0．154x（R=0．9921），线性范围在1～25μg之间，RSD为3．0％。The substituted hyantoins at C5,5 （5,5-dimethyl-hyantoin and 5-methyl-5-ethyl-hyantoin） were analyzed by fast atom bombardment-mass spectrometry（FABMS） with glycerin as marix. It was found that the intensities of their quasi-ion molecule are decreased exponentially with the equation I = a ＋ be^-kt, where a and b are constants related to ionization mechanism; t is time which is expressed by scan number in this experiment. It was also found that the ratio of initial intensities of quasi-ion molecules is only related to sample＇s concentration. When selecting any of them as internal standard, another was able to be analyzed quantitatively. In this work, 5,5-dimethyl-hyantoin was determined quantitatively using 5-methyl-5-ethyl-hyantoin as the internal standard. The standard equation is y = -0.0204 ＋ 0.154x（R = 0.9921）, the linear detection range is 1 - 25 μg, RSD is 3.0%.

关 键 词：快原子轰击质谱 非线性拟合 海因 

分 类 号：O657.63[理学—分析化学]