苯丙醇对映体在酰胺型手性固定相上的分离  

Enantiomer Separation of Phenlypropanol Using Amide Type Chiral Stationary Phases

作　　者：孙娟[1] 兰先秋[1] 唐小海 徐旭[1] 宋航[1] 

机构地区：[1]四川大学制药与生物工程系,成都610065 [2]成都药友科技有限公司,成都610041

出　　处：《分析化学》2006年第U09期158-160,共3页Chinese Journal of Analytical Chemistry

基　　金：四川省应用基础研究基金的支持(No.03JY029-058-1)

摘　　要：对于手性对映体苯丙醇在3种Pirkle型手性固定相(CSP)Whelk-O1、DNB-Leu和DNB-Pg上直接分离进行了研究。考察了流动相极性以及温度对分离的影响,发现在常用的范围内无法获得分离,仅有Whelk-O1在流动相极性的极端条件下(正己烷/异丙醇=99·5/0·5,V/V)能够被直接分离。在较低温度0℃和流速1mL/min的优化条件下能获得基线分离。用旋光检测器与液相色谱串联的方式确定了手性拆分结果,并确定了(-)苯丙醇优先洗脱的顺序。针对这种流动相极性极端条件下的分离,结合有关理论,进一步探讨了分离机理。The direct enantiomer separation of phenlpropanol on three Pirlk types of ehiral column Whelk-O 1, DNB-Leu, DNB-Pg had been studied. The influence of fluid polarity and temperature was investigated. It found only Whelk-O1 CSP could work in extreme condition of fluid polarity （Vhexane：V2-propanol =99.5：0.5）. Under the condition of lower temperature 0 ℃ and fluid rate 1 mL/min, the base-line separation can be obtained. The separation was further confirmed with HPLC linked with on-line OR spectrometry. （-）-Phenylpropanol was the 1 st eluting enatiomer. The effect of lower concentration of 2-propanol and the separation mechanism were discussed.

关 键 词：手性分离 苯丙醇 手性固定相 液相色谱/旋光检测器 

分 类 号：R917[医药卫生—药物分析学]