生物分子的毛细管电泳-化学发光检测法研究  

Separation and Determination of Biomolecules by Capillary Zone Electrophoresis-Chemiluminescence

作　　者：徐向东[1] 胡涌刚[1] 李欣欣[1] 

机构地区：[1]华中科技大学环境科学与工程学院

出　　处：《分析化学》2006年第U09期260-262,共3页Chinese Journal of Analytical Chemistry

基　　金：国家自然科学基金资助项目(No.20005005)

摘　　要：根据某些氨基酸和蛋白质在碱性条件下具有能显著增强鲁米诺-铁氰化钾体系化学发光信号的特性,对毛细管电泳-化学发光法直接分离测定生物分子的可行性进行了研究。电泳所用缓冲溶液为2·5mmol/L、含2·0mmol/L鲁米诺的硼砂缓冲溶液;氧化试剂为2·0×10-4mol/L铁氰化钾。在优化条件下,L-精氨酸和胃蛋白酶可直接实现分离,其检出限(S/N=3)分别为1·3×10-4mol/L和7·1×10-7mol/L。通过对市售的3种L-精氨酸标准样品进行分离分析研究,证明该方法可应用于L-精氨酸标准样品的纯度分析。A novel method for the analysis of biomolecules was developed by capillary zone electrophoresis-chemiluminescence（CZE-CL） based on the fact that some amino acids and proteins could catalyze the chemiluminescence reaction of Luminol-K3[Fe（CN）6] system. In addition, 2.5 mmol/L sodium tetraborate 2×10^-3 mol/L luminol and 2×10^-4 mol/L potassium ferricyanide （pH = 13.3） were chosen as optimum separation and detection condition for the determination of L-arginine and pepsin. The detection limits （S/N = 3） for L-arginine and pepsin were 1.3×10^-4 mol/L and 7.1×10^-7 mol/L, respectively. The presented method was used for the analysis of three real L-arginine samples successfully.

关 键 词：毛细管电泳 化学发光 生物分子 

分 类 号：O658.9[理学—分析化学] O657.3[理学—化学]