新型四羧基铝酞菁-二氧化硅荧光纳米粒子的合成及在酸度测定中的应用  

Preparation of Novel Tetra-Substituted Carboxyl Aluminum Phthalocyanine-Silica Fluorescent Nanoparticles and Application to Acidity Measurements

作　　者：陈小兰[1] 邹健莉[1] 赵婷婷[1] 黎中宝[2] 

机构地区：[1]厦门大学化学化工学院化学系,厦门361005 [2]集美大学水产学院,厦门361021

出　　处：《分析化学》2006年第11期1611-1614,共4页Chinese Journal of Analytical Chemistry

基　　金：厦门市科技创新项目(No.3502Z20022005)资助

摘　　要：首次制备出红区荧光染料四羧基铝酞菁掺杂的二氧化硅纳米粒子，并对其进行了表征。将环己烷、正己醇和表面活性剂Triton X-100按一定体积比（12．3：1．04：1）混合均匀，形成清澈透明的溶液；将适量的四羧慕铝酞菁溶解到浓氨水中，加入到上述混合溶液中，形成反向胶束。搅拌10min后加入一定量的四乙氧基硅烷，加快搅拌速度，促使四乙氧基硅烷进入反相胶束中的“纳米水池”，在碱性条件下，四乙氧基硅烷水解形成二氧化硅纳米粒子。采用该方法制备的核壳荧光纳米颗粒荧光稳定性强，生物相容性高，抗干扰能力强。将一定量四羧基铝酞菁掺杂的二氧化硅纳米粒子溶于水溶液中，随溶液pH值的增加，荧光强度增强，并在pH5．02，11．98的范围内，荧光强度与溶液酸度有良好的线性关系。该法已成功地用于自来水和模拟生物体系中pH的测定。预期该技术有望用于细胞内H^＋的实时监测。Novel fluorescent nanoparticles, entrapping water-soluble red-region fluorescent dye of tetra-substi- tuted carboxyl aluminum phthalocyanine （AIC4Pc）, have been synthesized and characterized. Adding an appropriate amount of AIC4Pc and concentrated ammonia water into the microemulsions prepared by mixing 1.00 mL of Triton X-100, 12.30 mL of cyclohexane, 1.04 mL of n-hexanol to form the reverse micelle, after stirring for 10 min the mixture was then added tetraethoxysilane, vigorously stirring to impel tetraethoxysilane diffused into the nanoreactor. Under the catalysis of ammonia, tetraethoxysilane hydrolysed and polymerized to form the silica nanoparticles. These nanoparticles had unique advantages of high fluorescence, good photosta- bility, and biocompatibility. The possibility of using the fluorescent nanoparticles as new fluorescent indicator for pH determination was also investigated. It was found that the fluorescence nanoparticles are pH sensible and the pH response range is between 5.02 and 11.98. The proposed method has been successfully applied to the determination the pH of running water and simulating biologic system. The method promises to be used to in-vivo, rate-time determination of H^＋ in cell.

关 键 词：荧光纳米粒子 四羧基铝酞菁 反向胶束 酸度测定 

分 类 号：O613.72[理学—无机化学]