人卵巢组织两种超低温冻存方法的研究  

Research of Two Methods for Human Ovarian Tissues Cryopreservation

作　　者：李宇彬[1] 周灿权[1] 杨国奋[1] 王琼[1] 麦庆云[1] 

机构地区：[1]中山大学附属第一医院妇产科生殖中心,广东广州510080

出　　处：《中山大学学报（医学科学版）》2006年第6期704-708,共5页Journal of Sun Yat-Sen University:Medical Sciences

基　　金：卫生部临床重点专科([2004]468);广东省科委科技攻关基金项目(2003A3020305);广州市科技计划资助项目(2004Z1-E0101)

摘　　要：【目的】探索两种人卵巢组织冷冻方案用于生殖细胞保存的效果。【方法】活检卵巢组织来自15名经知情同意的手术患者。组织被随机分配到新鲜组、玻璃化冷冻组和慢速冷冻组。观察和比较各组卵巢组织冻融后的原始卵泡形态完整率,并通过体外培养系统测定培养液中的雌二醇和孕酮水平,观察和比较冻融后卵巢组织内分泌功能。【结果】本研究中新鲜组、慢速冻融组和玻璃化冻融组的原始卵泡形态完整率分别是97.6%、72.6%和80.3%。两冻融组与新鲜组相比明显下降(P<0.001),而在两冻融组间差异并无统计学意义(P=0.237)。在体外组织培养期间,两种冻融卵巢组织都能持续分泌雌二醇和孕酮,两组间差异无统计学意义(P>0.05)。【结论】本研究中两种人类卵巢组织超低温冷存的方法是可行的,可适应不同需要。[Objective] The aim of this study was to explore the effects of two human ovarian tissue cryopreservation protocols for preserving germ cells. [Methods] Biopsies of ovarian tissue were collected from total 15 patients after informed consents. The tissues were randomly allocated to fresh, vitrification and conventional slow freezing groups. Morphological integral primordial follicles rates were compared between fresh and the post warming/ thawing tissue. Endocrinal functions of the post-cryopreservation tissue were also investigated and compared by estradiol and progesterone detections in in-vitro culture medium every other day. [Results] In this study, the morphological integral primordial follicles rates in the fresh, vitrification, and slow-freezlng groups were 97.6%, 80.3% and 72.6%, respectively. After warming/thawing, the rates significantly reduced in both freezing groups （P 〈 0.001）. No statistically significant differences in normal morphology primordial follicle rates were observed between the two cryopreservation approaches （P=0.237）, During in-vitro culture, estradiol and progesterone were secreted continuously by the both post-cryopreservation tissue pieces. And there were no statistical differences between the two groups （P 〉 0.05）. [Conclusions] Our study demonstrates that the two methods for human ovarian tissues cryopreservation are effective and can be fitted for different requires .

关 键 词：超低温冻存 卵巢组织 

分 类 号：R714[医药卫生—妇产科学]