前列腺增生患者逼尿肌中碱性成纤维细胞生长因子的变化  

Basic fibroblast growth factor in human detrusor muscle in bladder outlet obstruction due to benign prostatic hyperplasia

作　　者：韩增篪[1] 徐忠华[1] 刘犇[1] 阎磊[1] 周尊林[1] 刘海南[1] 刘照旭[1] 范医东[1] 陈修德[1] 郑宝钟[1] 

机构地区：[1]山东大学齐鲁医院泌尿外科,济南市250012

出　　处：《中华老年医学杂志》2006年第11期814-816,共3页Chinese Journal of Geriatrics

基　　金：山东省科技厅自然科学基金资助课题(Y2003C16)

摘　　要：目的探讨前列腺增生症患者膀胱逼尿肌中碱性成纤维细胞生长因子(bFGF)的表达及其意义。方法前列腺增生患者54例,分为逼尿肌稳定组(33例)和逼尿肌不稳定组(21例)；对照组15例为同期在我院因膀胱癌施行开放手术的男性患者。分别应用逆转录聚合酶链反应(RT-PCR)和免疫组化的方法检测各组逼尿肌中bFGFmRNA和bFGF蛋白的表达。结果稳定组和不稳定组bFGFmRNA的表达水平分别为0．5814±0．2362、0．6098±0．1829,明显高于对照组的0．3469±0．2986,差异有统计学意义(P＜0．05和0．01)；bFGF亦高于对照组。稳定组和不稳定组间差异无统计学意义(P＞0．05)结论前列腺增生患者致膀胱出口梗阻后膀胱逼尿肌细胞中bFGF表达水平升高,但与膀胱逼尿肌不稳定的发生无直接关系。Objective To investigate the change of the basic fibroblast growth factor （bFGF） leve in human detrusor muscle （DM） in bladder outlet obstruction （BOO） due to benign prostatic hyperplasia （BPH） and its implication. Methods Fifty four patients with BPH were divided into two groups： the obstructive DM stability and instability groups; and 15 men with bladder tumor who underwent operation in the same period were enrolled in the control group. The bFGF mRNA level in DM was measured by reverse transcription and polymerase chain reaction （RT-PCR） and the bFGF protein level was measured by immunohistochemical staining method. Results The bFGF -mRNA expression level of bladder smooth muscle cells was significantly lower in the control group than that in the obstructive DM stability and instability groups （all P 〈 0.05 ）, but there was no significant difference between the obstructive DM stability and instability groups（P〉0.05）. Conclusions The expression level of bFGF mRNA in bladder DM is elevated in BOO due to BPH, but there is little or no correlation between the increased expression of bFGF mRNA and detrusor muscle instability.

关 键 词：前列腺增生 成纤维细胞生长因子2 膀胱 

分 类 号：R697.3[医药卫生—泌尿科学]