荚膜组织胞浆菌素纯蛋白衍生物的制备  

Preparation of Purified Protein Derivative of Histoplasmin

作　　者：陈保文[1] 沈小兵[1] 苏城[1] 王国治[1] 

机构地区：[1]中国药品生物制品检定所菌苗室,北京100050

出　　处：《中国生物制品学杂志》2006年第6期617-618,622,共3页Chinese Journal of Biologicals

基　　金：国家高技术研究发展计划(863计划)资助项目(编号:2004AA2Z3452).

摘　　要：目的研制荚膜组织胞浆菌素纯蛋白衍生物。方法采用液体表面膜培养方法培养荚膜组织胞浆菌,培养液经盐析和弱酸沉淀法纯化,再经透析和除菌,制成荚膜组织胞浆菌素纯蛋白衍生物原液,并进行豚鼠的皮肤反应试验和安全性检测。结果荚膜组织胞浆菌素纯蛋白衍生物的蛋白纯度达80%以上,其1μg/ml的浓度诱导的豚鼠皮肤反应与国外同类制品等效,且具有良好的安全性。结论荚膜组织胞浆菌素纯蛋白衍生物可作为体内诊断制品,用于检查人体荚膜组织胞浆菌的感染以及荚膜组织胞浆菌病的临床诊断。Objective To prepare purified protein derivative of histoplasmin. Methods Culture Histoplasma capsulatum on the surface of liquid,collect the culture liquid,purify by salting out,precipitation by trichloroacetic acid,then dialyze and sterilize to prepare the bulk of purified protein derivative of histoplasmin. Perform skin and safety tests on the bulk in guinea pigs. Results The purified protein derivative of histoplasmin showed good safety and reached a purity of more than 80%. The skin reaction in guinea pigs induced by 1 μg/ml of purified protein derivative of histoplasmin was equivalent to that induced by the imported product of the same kind. Conclusion The purified protein derivative of histoplasmin might be used as a in vivo diagnostic reagent for infection of Histoplasma capsulatum and for histoplasmosis in clinic.

关 键 词：荚膜组织胞浆菌素 纯蛋白衍生物 皮肤反应 

分 类 号：R756.6[医药卫生—皮肤病学与性病学]