繁茂膜海绵细胞内微生物多样性的研究  

Phylogenetic diversity of endocellular bacteria marine sponge Hymeniacidon perleve

作　　者：靳艳[1] 郭鹏飞[1] 孙黎明[1] 虞星炬[1] 张卫[1] 

机构地区：[1]中国科学院大连化学物理研究所海洋生物产品工程组,大连116023

出　　处：《微生物学报》2006年第6期875-878,共4页Acta Microbiologica Sinica

基　　金：国家"973项目"-重点基础研究发展计划(2003CB716001)~~

摘　　要：采用海绵组织离散、细胞分离的方法,对繁茂膜海绵细胞进行纯化、胞内微生物DNA提取,构建了繁茂膜海绵细胞内微生物的16SrDNA克隆,对其遗传多样性进行了分析,发现海绵细胞内微生物16SrDNA序列主要归类于紫硫细菌门(Proteobacteria)中的α-亚门、γ-亚门和浮霉菌门(Planctomycetes)等类群。与研磨直接提取海绵组织DNA所得海绵组织中总微生物多样性相比,海绵细胞内存在丰富的浮霉菌(23%),说明浮霉菌主要存在于海绵细胞胞内。Marine sponges are hosts of diverse bacteria that live in both intracellular and intercellular spaces of the multicellular animals. The aim of this study is to investigate the bacteria diversity inside the marine sponge cells of Hymeniacidon perleve by 16S rDNA gene sequences. To obtain pure sponge cells, a protocol has been developed in which the sponge tissues were firstly dissociated in CMFSW and cleaned several times. The purified sponge cells were subject to extraction of endocelluar bacterial DNA. The endocellular bacterial phylogenetic diversity of the marine sponge was determined by RFLP-16S rDNA sequencing of cloned DNA fragments. Thirteen of isolated 16S rDNA gene sequences were attributed to be α-Proteobacteria （5）, γ- Proteobacteria （5） and Planctomycetes （3）. When compared to the bacterial diversity of the sponge tissues, α- and β-proteobacteria are still the dominant bacteria genes, however Planctomycetes was not obtained in the sponge tissuse. These results indicated a different bacterial diversity in the sponge cells and sponge tissues.

关 键 词：繁茂膜海绵 16S RDNA序列 胞内微生物 多样性 

分 类 号：Q938.8[生物学—微生物学]