花烛离体培养叶色变异株系的相关性状  

Characteristics of Chimeras of Anthurium andraeanum from in vitro Mutation

作　　者：徐彬[1] 辛伟杰[1] 王广东[1] 郭维明[1] 文方德 金剑平 

机构地区：[1]南京农业大学园艺学院,南京210095 [2]珠海市园艺研究所,珠海519070

出　　处：《植物学通报》2006年第6期698-702,共5页Chinese Bulletin of Botany

基　　金：国家自然科学基金(No.30300244);广东省科技厅项目(No.K130532002-1-4)

摘　　要：以花烛(Anthuriumandraeanum)间接器官发生途径中再生出的一株花叶变异植株为原始材料,进行增殖并对得到的3个叶色变异株系的叶色相关性状进行了初步研究。结果表明:通过愈伤组织器官发生途径和腋芽增殖途径对这一花叶苗进行增殖,均分离到3种变异株系,即花叶苗、黄化苗和天鹅绒绿色叶片苗;天鹅绒绿色苗叶片中的叶绿素含量比正常离体苗的含量低;叶片解剖结构表明,叶绿体在叶肉细胞中的分布与其叶片表现型相同,天鹅绒绿色叶片与正常叶片在解剖结构上无明显差异。花烛原套只具有1层细胞,无明显的L2层分生结构,因此叶肉的薄壁细胞完全由向各个方向分裂的原体细胞发育而来,这种组织结构导致花叶叶片中含有叶绿体的细胞和不含有叶绿体的薄壁细胞呈不规则分布。这种花叶株系可以作为育种材料或直接作为盆栽花烛进行推广。One variegated plantlet resulting from chlorophyll loss of partly parenchymal tissue was obtained from the adventitious shoots regenerated from callus of Anthurium. Three types of chimeras were achieved in the process of propagation by indirect shoot regeneration via callus or axillary bud emergence, namely variegated types, albino types, and jade-green types, in which the chlorophyll content was lower than that of the normal in vitro plantlets. Anatomy structure of leaves indicated the consistency of phenotypes and chlorophyll distribution but little difference between the jade-green leaf and the normal leaf. One cell layer constitutes the tunica, and thus no L2 layer can be defined. So all the parenchymal cells were derived from meristem cells in corpus, which divided in all directions. This tissue structure explains the irregular distribution of parenchymal cells containing chloroplasts or not. The chimeras, especially the variegated plantlets, might be potential breeding materials or new cultivars.

关 键 词：花烛 叶色变异 叶绿素含量 解剖结构 

分 类 号：S682.19[农业科学—观赏园艺]