盐酸关附甲素在五指山小型猪急性缺血模型中的电生理学作用  

Electrophysiological effects of acehytisine hydrochloride in a porcine model of acute coronary occlusion

作　　者：梁岩[1] 朱俊[1] 杨艳敏[1] 李建冬[1] 孙妍[1] 关键[1] 高鑫[1] 李长瑞[1] 唐跃[2] 

机构地区：[1]中国医学科学院中国协和医科大学阜外心血管病医院急症抢救中心,北京100037 [2]中国医学科学院中国协和医科大学阜外心血管病医院动物实验中心,北京100037

出　　处：《中华心血管病杂志》2006年第11期1035-1039,共5页Chinese Journal of Cardiology

基　　金：国家"863"课题滚动基金的一部分

摘　　要：目的研究分析盐酸关附甲素(AHH)对结扎冠状动脉(冠脉)造成急性缺血的五指山小型猪的心电图及心内电生理指标影响。方法选用五指山小型猪,随机分为A组(结扎冠脉并应用AHH)、B组(不结扎冠脉仅应用AHH)、C组(结扎冠脉而应用生理盐水)三组,分别记录急性缺血(或对照)动物用药前后心房、房室交界区、心室电图及体表监测导联心电图。结果共收集五指山小型猪有效实验资料每组各9只,性别、月龄、身长、体重等基线资料组间差异无统计学意义。在急性缺血模型中与生理盐水比较,AHH延长P波及PR间期,缩短QTc间期,并延长心房不应期及室房逆传,缩短心室不应期。与非缺血模型比较,应用AHH在急性缺血模型中出现QT及QTc间期缩短,心室相对不应期及功能不应期缩短,室房逆传有效不应期延长。实验过程中,无论急性缺血或非缺血模型,应用AHH均未发现新的心律失常。结论初步动物实验结果证实,在急性缺血模型中,AHH表现出一定的电生理作用而未引发新的心律失常。Objective To observe the electrophysiological effect of Acehytisine Hydrochloride （AHH） in Wu Zhi Shan（WZS） micropigs with experimental acute coronary occlusion. Methods Adult WZS micropigs were randomized into group A： coronary ligation with AHH infusion （n = 9） ; group B： AHH infusion without coronary ligation （n =9） and group C： coronary ligation with saline （NS） infusion （n = 9）. Surface ECGs and cardiac electrophysiological data including atrium, atrium-ventricle junction and ventricle electrograms were collected by programmed electrical stimulation at ischemic baseline and after AHH （or NS） infusion. Results Compared to animals treated with AHH without ischemia, VARC-ERP was significantly increased while QT, QTc intervals, VRRP and VFRP were significantly reduced in ischemic animals treated with AHH. Compared to ischemic animals treated with saline, AHH prolongs the P-wave duration and PR interval, shortens QTc interval, prolongs ARP and AEP, also prolongs V-A reverse conduction time and VARC-ERP but shortens VFRP. No proarrhythmia effect was found in both AHH treated groups. Conclusion AHH resulted in significant electrophysiological effects on this porcine acute coronary ischemic model.

关 键 词：盐酸关附甲素 心肌缺血 电生理学 

分 类 号：R285.5[医药卫生—中药学]