乳糖诱导植物甜蛋白des-pGlu1-Brazzein在大肠杆菌中的表达  

The Influences of Lactose as an Inducer on Expression of Plant des-pGlu1-Brazzein in Escherichia coli

作　　者：李春丽[1,2] 陈其新[1,3] 何国庆[4] 

机构地区：[1]河南农业大学牧医工程学院 [2]浙江大学生物系统工程与食品科学学院,杭州310019 [3]甘肃亚盛集团博士后科研工作站北京分站,北京100101 [4]浙江大学生物系统工程与食品科学学院

出　　处：《生物工程学报》2006年第6期1021-1025,共5页Chinese Journal of Biotechnology

基　　金：河南省教育厅基础研究项目(No.2004922043);河南农业大学博士后基金资助。~~

摘　　要：构建了植物甜蛋白des-pGlu1-Brazzein高效表达的工程菌株,对其乳糖诱导条件进行了优化。乳糖浓度、诱导时间和诱导温度对菌株生长和目的蛋白表达的试验结果显示,高浓度乳糖对菌株生长有抑制作用(P<0.01),对目的蛋白的表达无显著影响(P>0.05),时间延长对于菌株生长有利(P<0.01),对目的蛋白的表达因温度不同而不同。进一步的研究表明,以0.5%乳糖在28℃～30℃诱导4h对菌株生长和目的蛋白的表达有利,目的蛋白的表达量可达20%左右。Expression strain of des-pGlul-brazzein was constructed and the conditions using lactose as inducer was also optimized. The Influences of three factors which were lactose concentration, induction time and inducing temperature on the growth of strain and on the yield of des-pGlul-Brazzein was analyzed in detail. The result indicated that high lactose concentration inhibit the growth of strains（ P 〈 0.01） but made no difference on expression of target protein between 0.5% - 5% （ P 〉 0.05）, Biomass would be improved as time passed（ P 〈 0.01） ,but the yield of target protein didn＇ t increase obviously at 30℃ compared with at 37℃. Further result showed that the greater expressed level of des-pGlul-Brazzein, as high as about 20% of total cell protein, could be achieved after the strain had been induced with 0.5% lactose under 28℃- 30℃ for 4h.

关 键 词：植物甜蛋白 大肠杆菌 乳糖 优化 

分 类 号：Q786[生物学—分子生物学]