大规模酵母双杂交技术研究蛋白质相互作用的应用  

The Application of Large-scale Yeast Two-hybrid to Study Protein—Protein Interaction

作　　者：吴志豪[1] 王建[1] 贺福初[1] 

机构地区：[1]军事医学科学院放射与辐射医学研究所,北京100850

出　　处：《遗传》2006年第12期1627-1632,共6页Hereditas（Beijing)

基　　金：国家高技术研究发展计划项目(863计划)(编号:2002-BA711A11;2004BA711A19);北京市科委计划项目(编号:H030230280290)~~

摘　　要：简介了酵母双杂交技术原理,总结了酵母双杂交技术大规模筛选蛋白质相互作用的基础、应用及存在的问题。因为大规模酵母双杂交技术结果有大量假阳性及假阴性问题,因此,有条件情况下有必要同时开展其他方法的大规模蛋白相互作用研究,以构建规模更大可信度更高的蛋白质相互作用网络图。The principle of yeast two-hybrid were summarized, the basis for large scale yeast two-hybrid screening of protein-protein interaction was given and the main application of yeast two-hybrid in large scale screening of protein-protein interaction was presented. The problems of large scale yeast two-hybrid screening of protein-protein interaction were also discussed. Because there were a lot of false positives and false negatives in the result of protein-protein interaction obtained by large-scale yeast two-hybrid screening, the employment of other methods to study protein-protein interaction in large scale in parallel was proposed if possible.

关 键 词：大规模 酵母双杂交 蛋白质相互作用 

分 类 号：Q78[生物学—分子生物学]