激肽原基因的选择性剪接与功能研究进展  

Progress in the Study on Alternative Splicing and Functions of Kininogen Genes

作　　者：周丽伟[1] 马飞[1] 李庆伟[1] 

机构地区：[1]辽宁师范大学海洋生物功能基因与蛋白质组学研究所,大连116029

出　　处：《遗传》2006年第12期1649-1655,共7页Hereditas（Beijing)

基　　金：国家自然科学基金(编号:60305001;60575005);辽宁省教育厅青年基金(编号:2004F090);大连市优秀青年科技人才基金项目资助~~

摘　　要：激肽原基因在不同物种中有一定的差异,其编码的蛋白质产物也有所不同。在人类、牛和小鼠中存在的激肽原基因K是一种选择性剪接基因,其可以编码两种激肽原产物,而在大鼠中,除了激肽原基因K之外还有一类激肽原基因T,其只能编码一种激肽原。激肽原属于半胱氨酸蛋白酶抑制剂超家族中家族3的成员,是一类具有多个结构域的多功能蛋白,在人体内多种功能协同作用,维持着机体的正常生理状态。激肽原的凝血和抗凝血两种相互拮抗的功能既能使破损的血管得到修复,防止流血不止,又能抑制血管内血栓的形成。本文将对目前研究较多的人、牛、小鼠和大鼠的激肽原基因的选择性剪接以及激肽原的凝血、抗凝血等多方面的功能做一综述,为更进一步地阐明激肽原基因的进化机制,加深对基因选择性剪接机制的了解,揭示激肽原功能的认识等方面有所启示,并为调节血管通透性、调节血压和抗肿瘤等新药的开发提供有益的参考。The kininogen gene and its coded proteins are slightly dffferent in various species, in human beings, bovine and mouse, for example, there is an alternatively spliced kfninogen gene K, encoding two kinds of proteins. By contrast, there is still another constituted spliced kininogen gene T which encodes only one kind of protein in the rat. The kininogen, belonging to the family 3 of cystatin supeffamily, is a kind of multffunctional proteins with multiple domains, which maintains the normal physiological condition in human and some other organisms. The antagonism of hemoglutination and antfhemoglutinatron of kininogen can not only recuperate the damaged blood vessels to prevent them from bleeding ceaselessly, but also restrafn the formation of thrombus. In this review, we briefly discussed alternative splicing of kininogen gene and the multffunction of kininogen protein, as well as prfmarily hemoglutination and antihemoglutination, in human beings, bovine, mouse and rat which have been currently studied in detail. Our aims are to provide the beneficial references for further understanding the mechanism of evolution and alternative splicing of kininogen gene, and elucidating the multifunction roles new medicines to regulate the vascular permeability and of kininogen protein. Besides, it would be helpful for developing blood pressure and to restrain tumor.

关 键 词：激肽原 选择性剪接 抗凝血 凝血 疾病 

分 类 号：Q75[生物学—分子生物学]