氯沙坦对糖尿病大鼠肾脏细胞因子表达的影响及意义  

Impact of Losartan on cytokins expression in the renal of STZ-induced diabetic rats

作　　者：柳刚[1] 关广聚[1] 亓同钢[2] 赵敬杰[2] 傅余芹[1] 李学刚[1] 吴涛[1] 文蓉珠[1] 

机构地区：[1]山东大学第二医院肾内科,250033 [2]山东大学第二医院分子生物学实验室

出　　处：《Journal of US-China Medical Science》2005年第3期47-50,57,共5页美中医学（英文版）

基　　金：国家教育部博士点基金资助项目（No.20030422068）,山东省中医药管理局资助项目（No.200329105）

摘　　要：目的探讨氯沙坦治疗糖尿病肾病的作用及其机制。方法采用单侧肾切除的STZ诱导的糖尿病大鼠，观测其肾脏形态、功能的变化。同时，采用荧光实时定量RT-PCR方法检测TGF—β1、CTGF、PAI-1等细胞因子在糖尿病大鼠肾脏中的表达情况。结果与正常组（NC组）相比，8周时糖尿病肾病组（DN组）大鼠的肾脏肥大指数（KW／BW）、平均肾小球体积（VG）、尿白蛋白排泄率（UAER）、肌酐清除率（Ccr）均升高。DN组肾皮质CTGF、PAI—1、FN表达水平也显着增高（p〈0．05）；与DN组相比较，氯沙坦治疗组的上述指标则均明显下降（p〈0．05）。结论氯沙坦可通遏抑制TGF—β1、CTGF、PAI-1等细胞因子的表达，对糖尿病人鼠肾脏起到保护作用。Objective To explore the effects of Losarum on renal structure and function in diabetic rats. Methods Male Wistar rats which underwent unilateral nephrectomy were randomly divided into 3 groups： normal control group （NC）, streptozotocin-induced diabetic group（DN） and diabetic group treated with Losartan （DNL）.Urinary albumin excretion rate（UAER）, creatinine clearance rate（Ccr）, left kidney weight/body weight ratio（KW/BW） and glomerular volume（VG） were observed. The expression of transforming growth factor-betal （TGF-β1）, connective tissue growth factor（CTGF）, fibronectin（FN） and plasminogen activator inhibitor-1（PAI-1） were tested by real-time quantitative PCR at the end of the experiment. Results UAER, Ccr, VG, and KW/BW were elevated significantly in group DN in the 8th week after the induction of diabetes. The expressions of TGF- β 1, CTGE PAI-1 and FN in group DN were also increased significantly as compared with group NC, while all of these indices were decreased in DNL group（P 〈 0.05 =. Conclusion Losartan could protect the STZ-induced diabetic rats from diabetic nephropathy by suppressing the over-expression of TGF- β1, CTGF, PAI-1 and FN.

关 键 词：糖尿病肾病 转化生长因子 结缔组织生长因子 纤溶酶原激活物抑制剂-1 

分 类 号：R144.1[医药卫生—公共卫生与预防医学]