小儿肾病综合征血清皮质醇及T细胞亚群检测的临床意义  

Clinical significance of meassuring serum cortisol and T lymphocyte subsets in children with nephrotic syndrome

作　　者：熊建平[1] 何念海[1] 

机构地区：[1]第三军医大学西南医院儿科,重庆400038

出　　处：《重庆医学》2006年第22期2023-2024,共2页Chongqing medicine

摘　　要：目的通过测定肾病综合征小儿血清皮质醇和T细胞亚群来探索小儿肾病综合征血清皮质醇水平与T细胞亚群的关系及其临床意义。方法采用放射免疫分析的方法测定小儿血清皮质醇水平,同时应用单克隆抗体技术检测小儿的T细胞亚群。结果实验两组血清皮质醇水平差异无统计学意义(P>0.05);然而肾病小儿CD3+、CD4+以及CD4+/CD8+均明显低于正常对照组小儿(P<0.05),肾病组CD8+比例明显高于正常对照组(P<0.05)。结论肾病小儿糖皮质激素水平虽然基本正常,但其受体数量下调,细胞免疫功能受损,这表明T细胞亚群可作为肾病综合征活动性的一个指标。Objective To study the clinical significance of meassuring serum cortisol and T lymphocyte subsets, and to explore the relationship between serum cortisol and T lymphocyte subsets in children with nephrotic syndrom（NS）. Methods Serum cortisol was meassured by radioimmunoassay and T lymphocyte substs were determined by monoclonal antibody technique. Results The contents of serum cortisol had no difference in two groups（P〉0.05） ;however the percentages of CD3^＋ cell, CD4^＋ cell and the ratio of CD4^＋ /CD8^＋ were significantly lower in children with NS than in normal control group（ P〈0.05）, the percentages of CD8^＋ cell was significantly higher in children with NS than in normal control group（P〈0.05）, Conclusion Because of the normal content of serum cortisol, their cellularimmunity is impaired while the quantity of glucocorticosteroid receptor（GCR）in patients with NS is down regulation, It indicates that T lymphocyte subsets can be used as an index of activity in nephrotic syndrome.

关 键 词：肾病综合征 血清皮质醇 T细胞亚群 

分 类 号：R726.91[医药卫生—儿科]