灰树花糖蛋白中总糖含量的测定  

Determination of Total Sugar in Glycoprotein of Grifola frondosa

作　　者：杨晓华[1] 于广利[1] 赵峡[1] 宋乐天[1] 高昊东[1] 

机构地区：[1]中国海洋大学海洋药物与食品研究所,山东青岛266003

出　　处：《中国海洋大学学报（自然科学版）》2006年第6期929-931,共3页Periodical of Ocean University of China

基　　金：国家自然科学基金项目(30370345)资助

摘　　要：探讨糖蛋白中总糖含量测定的简捷方法,以2种灰树花糖蛋白(GF6A,GF6B)为原料,分别以硫酸-苯酚法和次亚碘酸钠法对其中的总糖含量进行了比较分析。结果显示,应用前者方法测定多糖含量分别为85.45%和38.46%,采用后者方法测定结果分别为87.03%,36.51%,2种方法测定的结果相近,其中以硫酸-苯酚法因不需对样品进行预水解,方法简便、可靠,更适合于糖蛋白中总糖含量测定。In this study, the total sugar contents of two glycoproteins （GF6A and GF6B） purified from Grifola frondosa were compared and analyzed by the sulfuric acid-phenol colorimetric method and the NaIO titration method. The experimental results showed that the total sugar contents of GF6A and GF6B were 85.45 % and 38.46%, respectively by the sulfuric acid-phenol method, and 87.03% and 36.51%, respectively by the NaIO method. The reliability and stability of the two methods were similar; however, compared to the NaIO method; the sulfuric acid-phenol method was faster and convenient, therefore it is more suitable for the total sugar analysis in glycoprotein.

关 键 词：灰树花糖蛋白 硫酸-苯酚 次亚碘酸钠 总糖 

分 类 号：Q936[生物学—微生物学]