山羊子宫内膜腺上皮细胞的分离培养和鉴定  

Isolation,Culture and Identification of Endometrium Glands in Goats

作　　者：丰艳妮[1] 吴庆侠[1] 吴瑞[1] 楚元奎[1] 靳亚平[1] 

机构地区：[1]农业部家畜生殖内分泌与胚胎工程重点开放实验室西北农林科技大学动物科技学院,陕西杨凌712100

出　　处：《西北农业学报》2006年第6期12-14,共3页Acta Agriculturae Boreali-occidentalis Sinica

基　　金：国家自然科学基金资助项目(39770545);西北农林科技大学青年骨干教师支持计划项目(01140305)

摘　　要：为了探讨山羊子宫内膜腺上皮细胞的分离、生长条件以及纯度鉴定，通过2g／L的Ⅰ型胶原酶置于水浴消化4h，200目的滤网过滤后进行低速离心，收集沉淀物，沉降洗涤3～5次，将细胞移至24孔培养板置37℃细胞培养箱中进行培养。12h后开始贴壁生长，腺团开始向外部扩散，生长2～3d，细胞扩散并出现明显的铺路石状，4～5d腺团基本扩散开来并呈一定走向继续生长。经免疫组织化学方法进行细胞染色，细胞表达角蛋白的阳性率达90％以上。This paper focus on find the mathod of endometrium glands＇ isolation, growth conditions and identification. Chipped the tissues into 1mm3, then digested 4 h with Collagenase Ⅰof 2g/L at 37. After filtered with 200 Sieve, gently centrifugalized and collected the sediments. Washing 3-5 times, then transfer them into 24 plate, cuture in 37℃. After 12 h, the glands begin to growth and extend, and become cobblestone 2 - 3 d later. ,For 4 - 5 d the glands fully extended and grow along certain way. Verified by immunocytochemical staining method, the results showed that the cells were positive for cytokeratin more than 90 % of positive rate.

关 键 词：子宫内膜 腺上皮细胞 免疫组织化学 鉴定 山羊 

分 类 号：Q813.11[生物学—生物工程]