辣椒抗枯萎病体细胞变异无性系筛选粗毒素适宜剂量的研究  

Study on the Proper Concentration of Crude Toxin for Somaclonal Variation Resistant to Capsicum Wilt

作　　者：李大伟 黄炜 巩振辉 

机构地区：[1]西北农林科技大园艺学院,陕西杨凌712100

出　　处：《西北农业学报》2006年第6期130-134,共5页Acta Agriculturae Boreali-occidentalis Sinica

基　　金：陕西省农业分子生物学重点实验室基金;西北农林科技大学植物遗传育种专项(05YZ024-1)资助

摘　　要：选用“农城椒2号”、“0127”、“0171”3个辣椒品种作为试材,从辣椒种子发芽、植株生长及愈伤组织诱导方面对辣椒枯萎病病原菌粗毒素生物活性进行了系统研究。结果表明,辣椒枯萎病病原菌粗毒素对辣椒种子发芽、植株生长及愈伤组织诱导具有明显的毒害和抑制作用,不同辣椒品种对粗毒素的抗性表现不同,并结合辣椒离体再生体系,确定了0.60 g/L为辣椒抗枯萎病体细胞变异无性系筛选粗毒素适宜剂量。The effect of the crude toxin derived from capsicum wilt pathogen was studied on the germination of seeds, growth of seedlings, elongation of radicel and induction of callus in three cultivars of pepper, ＇ Nongchengjiao 2＇,‘0127＇, and ‘0171 ＇. The result showed that the crude toxin of capsicum wilt pathogen can not only extremely inhibit both the germination of seeds and the growth of seedlings, but also do great harm to the elongation of radicel and induction of callus. Under the same concentration of crude toxin, the degree of damage was different from varied genotypcs. It can select somaclonal variation resistant to fusarium wilt of pepper at the concentration of 0.60 g/L of crude toxin properly.

关 键 词：辣椒 枯萎病 镰刀菌粗毒素 体细胞无性系 愈伤组织 

分 类 号：S436.418[农业科学—农业昆虫与害虫防治]