D3S1358 vWA D8S1179在汉族群体遗传多态性分析  

Genetic Polymorphism of D3S1358 vWA and D8S1179 in Han Population

作　　者：葛秀峰[1] 朱运良[1] 张红伟[2] 

机构地区：[1]郑州大学基础医学院法医学教研室,郑州市450052 [2]郑州大学附属第一医院外总实验室,郑州市450052

出　　处：《医药论坛杂志》2006年第21期34-35,共2页Journal of Medical Forum

摘　　要：目的研究D3S1358,vWA和D8S1179三个短串联重复序列(STR)基因座的等位基因频率在广东汉族群体内的分布,为这三个基因座的应用提供基础数据。方法用Chelex100法提取抗凝血的DNA,用PowerP lex(16系统扩增样品DNA,扩增产物经AB I PR ISM(377 DNA Sequencer电泳后,用genscan3.1和gen-otyper2.1软件确定各等位基因。统计各等位基因频率,用χ2方法检验遗传平衡吻合度。计算杂合度(H)、多态信息含量(PIC)、个人识别概率(DP)及亲权否定概率(PPE)。结果得到3个基因座各等位基因及其频率,分布符合遗传平衡定律,具有高的杂合度和多态信息含量。结论3个基因座具有较高的杂合度,是较理想的遗传标记,可用于法医学个人识别和亲权鉴定以及人类遗传学研究。Objective To investigate the allele frequencies of D3S1358, vWA and D8S1179 in Guangdong Han population and to provide basic data for their use in related field. Methods DNA extracted with Chelexl00 from anti -coagulative blood sample was amplified with PowerPlex 16 system. The PCR product was analyzed with ABI PRISM 377 DNA Sequencer and typed with genscan2.1 and genotyper3.1 software. After getting the allele frequencies and phenotype frequencies,X2 test was performed to determine if it follows Hardy - Weinberg＇s law. Based on the allele frequencies, the value of heterozygosity （H） , polymorphism information content （PIC） , discrimination probability （DP） and exclusion power （PPE） were calculated according to related formula. Results 3 allele frequencies were gotten and the allele distribution agreed with Hardy - Weinberg equilibrium. The genetic parameters, such as heterozygosity （H）, polymorphism information content（ PIC）, power of discrimination（ DP）, probability of paternity exclusion（ PPE）, were also calculated. Conclusion These 3 STR loci possessed high heterozygosity and can be used as genetic markers in human genetic study, personal identification and paternity test.

关 键 词：短串联重复序列 遗传多态性 个人识别 亲权鉴定 

分 类 号：Q347[生物学—遗传学]