HPLC/MS/MS法分离鉴定罗通定在大鼠胆汁中的代谢产物  

Identification of Rotundine Metabolites in Rat Bile by HPLC/MS/MS

作　　者：牟昀雅[1] 余伯阳[1] 

机构地区：[1]中国药科大学中药复方研究室,南京210038

出　　处：《中国天然药物》2006年第6期448-451,共4页

摘　　要：目的:研究罗通定在大鼠胆汁中的主要代谢产物。方法:灌胃给药后,收集大鼠胆汁,液液萃取,采用HPLC/MS/MS法分析鉴定罗通定的代谢产物。色谱柱Lichrospher-C18(5μm,4.6mm×250mm),流动相:甲醇-水-三乙胺(37630.063,V/V/V),冰醋酸调pH至6.3,检测波长281nm。结果:在大鼠胆汁中发现2个罗通定代谢产物,初步推测其结构为罗通定单羟基化后再与葡萄糖醛酸结合的产物。结论:罗通定在大鼠胆汁中主要以羟基化后的葡萄糖醛酸结合物形式排泄。AIM： To investigate the main metabolites of mttmdine in rat bile. METHOD： Bile of rats after oral administration of rotundine （200 mg·kg^-1） was analysed by HPLC/MS/MS. Chromatographic analysis was performed on a Lichrospher-Cl8 column （5μm, 4.6mm×250 mm）; mobile phase： methanol-water-triethylamine （37 ： 63 ： 0.063, V/V/V）, adjusting pH to 6.3 with acetic acid. UV detector was set at 281 nm and positive ion mode was operated. RESULT： Two metabolites were found in rat bile, whose structure may be glucuronide conjugates of rotundine after demethylation. CONCLUSION： Conjugate with glucuronic acid after demethylation may be the main excretion pathway of rotundine in rat bile.

关 键 词：罗通定 左旋四氢巴马汀 胆汁 代谢产物 LC/MS/MS 

分 类 号：R969.1[医药卫生—药理学]