几种粉螨同工酶比较及亲缘关系初探  

Comparison of Isozymes of Several Acari and Its Utilization in Polygenetic Affinities

作　　者：邹志文[1] 夏斌[1] 罗冬梅[1] 余丽萍[1] 朱志民[1] 

机构地区：[1]南昌大学生命科学学院,江西南昌330031

出　　处：《南昌大学学报（理科版）》2006年第5期484-488,共5页Journal of Nanchang University(Natural Science)

基　　金：国家自然科学基金资助项目(39860014)

摘　　要：以5种粉螨为实验材料,以普通肉食螨和桔全爪螨为对照,采用垂直板型聚丙烯酰胺凝胶电泳法(PAGE),对常见同工酶EST、MDH、ADH、CAT等进行比较研究。结果表明:不同种类粉螨间的酶带具有多样性,存在不同程度的相似相异性,为粉螨的分类鉴定提供了佐证,利用相似系数初步分析了几种粉螨间亲缘关系的远近。The activity and zymogram of EST,MDH ,AMY ,ADH ,CAT were studied in five Acari with Cheyletus eruditus and Panonychus citri as reference group by means of polyacrylamide gel electrophoresis. Results indicated that to some extent there were both some similarities and differences existed in the isozyme of the acari. The present study provided additional proof for the classification of acari and suggested different polygenetic affinities by studying the similarity coefficient of them.

关 键 词：粉螨 同工酶 聚丙烯酰胺凝胶电泳 亲缘关系 

分 类 号：Q55[生物学—生物化学]