促结肠动力中药筛选及其机制  

Selection of Chinese medicine with procontractile activity on colonic muscle and its mechanism

作　　者：衣兰娟[1] 田琳[1] 梁宁霞[1] 魏睦新[1] 

机构地区：[1]南京医科大学第一附属医院中医科,江苏省南京市210029

出　　处：《世界华人消化杂志》2006年第32期3093-3097,共5页World Chinese Journal of Digestology

基　　金：江苏省"135"工程重点人才项目;No.RC2002049

摘　　要：目的:探讨中药生白术、小茴香、蒲公英、莱菔子、大腹皮、莪术的促结肠动力作用,以筛选出具有开发价值的促结肠动力药物,并探讨其初步机制.方法:制备豚鼠结肠纵肌,以9g/L氯化钠溶液和乙酰胆碱为对照,观察6味中药对离体结肠肌条收缩活性的效应.实验结果以振幅(g)、时间(s)和曲线下面积(g·s)表示.分析对象为加药后5min内出现的最大波形.结果:该6味中药对肌条均有不同程度的兴奋效应,中药组(依次为大腹皮、莱菔子、蒲公英、小茴香、莪术、生白术)与生理盐水组相比,振幅峰值(0.97±0.40,0.95±0.26,0.87±0.49,0.85±0.26,0.78±0.39,0.60±0.25vs0.03±0.04,P<0.01)和曲线下面积(1.40±1.00,0.75±0.79,0.62±0.54,0.64±0.59,0.40±0.37,0.29±0.15vs0.11±0.06,P<0.05或P<0.01)均有显著性差异,以大腹皮作用最强,时间长达175±238.07s.在阿托品和维拉帕米分别预孵育情况下,加入各中药后与单独中药组的肌条收缩幅度增强的幅度明显减弱,两组间差异有显著性(中药+拮抗剂组vs中药组,P<0.05).结论:该6味中药对结肠平滑肌均有不同程度的兴奋效应,引起的收缩效应可被M受体阻断剂阿托品部分阻断,通过细胞外Ca2+内流介导.AIM： To determine the of six Chinese herbal procontractile activity medicines, including Rhizoma Atractylodis Macrocephalae （RAM）, Fructus Foeniculi （FF）, Herba Taraxaci （HT）, Semen Raphani （SR）, Pericarpium Arecae （PA） and Rhizoma Curcumae （RC） on the colonic muscle strips isolated from guinea pigs, and study the possible mechanisms. METHODS： The colonic longitudinal muscle strips of guinea pigs were prepared. Sodium chloride （9 g/L） and acetylcholine were used in the controls. The effects of six Chinese herbal medicines （RAM, FF, HT, SR, PA, RC） and the control medicine on the contraction of isolated colonic strips from guinea pigs were observed. The results were expressed with contraction amplitude （g）, contraction time, and area under curves （AUC, g · s）, and the maximal wave height was analyzed 5 min within the addition of drugs. RESULTS： The six Chinese herbal medicines （PA, SR, HT, FF, RC, and RAM, respectively） induced a significant elevation of colonic contractile response in comparison with normal saline did （contraction amplitude： 0.97 ± 0.40, 0.95 ± 0.26, 0.87 ± 0.49, 0.85 ± 0.26, 0.78 ± 0.39, 0.60 ± 0.25 vs 0.03 ± 0.04, P 〈 0.01; AUC： 1.40 ± 1.00, 0.75 ± 0.79, 0.62 ± 0.54, 0.64 ± 0.59, 0.40 ± 0.37, 0.29 ± 0.15 vs 0.11 ± 0.06, P 〈 0.05 or P 〈 0.01）, of which PA had the strongest effect, and the contraction time was 175 ± 238.07 s. Atropine and verapamil caused an evident suppression of colonic contraction induced by the Chinese medicines （P 〈 0.01）. CONCLUSION： All the six Chinese herbal medicines PA, SR, HT, FF, RC, and RAM have procontractile activity on colon smooth muscle, which is mediated by M receptor and depends on the presence of extracellular calcium.

关 键 词：中药 胃肠动力 平滑肌 结肠 豚鼠 

分 类 号：R285.5[医药卫生—中药学]