不同疾病进展阶段的HIV-1 B亚型毒株感染者对Gag、Nef肽段库的特异性CTL应答比例的比较和分析  

Comparison of recognition rates of Gag,Nef peptide pools in HIV-1 clade-B infected individuals with different stages of progress

作　　者：焦洋[1] 谢静[1] 韩扬[1] 邱志峰[1] 左玲燕[1] 王爱霞[1] 李太生[1] 

机构地区：[1]中国医学科学院中国协和医科大学北京协和医院感染内科,北京100730

出　　处：《中华微生物学和免疫学杂志》2006年第11期1022-1025,共4页Chinese Journal of Microbiology and Immunology

基　　金：首都医学发展科研基金艾滋病项目(2003-1006);欧盟艾滋病疫苗项目(ICA4-CT-2002-10042)

摘　　要：目的对不同疾病进展阶段的HIV-1B亚型毒株感染者Gag、Nef特异性细胞毒性T淋巴细胞(cytotoxic T lymphocyte,CTL)应答进行研究,比较和分析不同患者群对不同肽段库应答比例的异同,探讨针对不同肽段库的特异性CTL应答在延缓病程进展中所起的作用。方法选取56例未经抗病毒治疗的中国HIV-1B亚型毒株感染者。其中包括长期无进展者(long-term nonprogressors,LTNP)、HIV感染早中期患者和AIDS患者3组不同疾病进展阶段患者。以覆盖HIV-1 Gag全长和部分Nef的14个肽段库为刺激原,应用IFN-γELIDSPOT法测定3组患者的特异性CTL应答,并比较3组患者对不同肽段库的应答比例。结果3组不同进展阶段患者对14个肽段库总体的特异性CTL应答的平均反应宽度和强度间差异均无统计学意义。3组患者对14个肽段库的识别模式可分为两种类型：(1)对Gag-p24-1、Gag-p24-5、Gag-p2/7/1/6-1以及Gag-p2/7/1/6-3这4个肽段库的识别比例高低与病情进展情况相平行。3组患者对4个肽段库整体的识别比例间差异有统计学意义(P=0．041)；(2)对其他10个肽段库的识别与病情进展不平行,在HIV感染早中期患者中比例高,而在LTNP中低。结论针对不同肽段库的CTL应答可能在控制病毒复制过程中发挥不同的作用,对疾病进展的控制可能需要针对多个抗原表位的有效CTL应答。Objective To study the characteristics of HIV-1 specific cytotoxic T lymphocyte （CTL） responses in Chinese HIV-1 clade-B infected individuals with different stages of progress and to evaluate the roles of the specific CTL responses to different peptide pools in deferring disease progression. Methods HIV-1 specific CTL responses to 14 overlapping peptide pools encompassing products of the HIV-1 Gag and a part of Nef genome were tested through INF-γ enzyme-linked immunospot（ELIPOT） assays in 56 HIV-1 clade-B infected individuals and the recognition rates of these peptide pools were compared among three cohorts, including long-term nonprogressors（LTNP）, individuals with asymptomatic infection and AIDS patients. Results No significant differences were found among the average frequencies and breadths of HIV-1 specific CTL responses of the three cohorts. Two patterns were found in the recognition of the 14 peptide pools when comparing the recognition rates among the three cohorts. The recognition rates of Gag-p24-1, Gag-p24-5, Gag-p2/7/1/6-1 and Gag-p2/7/1/6-3 among the three cohorts were parallel to their disease progression. When comparing the recognition of the total 4 peptide pools, a significant differenee was found among the rates of the three eohorts（ P =0.041） . Conclusion CTLresponses to different peptide pools may play different roles in suppressing viral replications and broadly directed HIV-1-specific CTL responses may also be necessary in deferring disease progression.

关 键 词：人免疫缺陷病毒1型B亚型 细胞毒性T淋巴细胞 长期不进展者 

分 类 号：R392[医药卫生—免疫学]