Interaction between hydrogen sulfide/cystathionine γ-lyase and carbon monoxide/heme oxygenase pathways in aortic smooth muscle cells  

作　　者：Hong-fang JIN Jun-bao DU Xiao-hui LI Yan-fei WANG Yin-fang LIANG Chao-shu TANG 

机构地区：[1]Department of Pediatrics, Peking University First Hospital, Beijing 100034, China [2]Institute of Cardiovascular Research, Peking University First Hospital, Beijing 100034, China

出　　处：《Acta Pharmacologica Sinica》2006年第12期1561-1566,共6页中国药理学报（英文版）

基　　金：Project supported by the National Natural Science Fundation for Distinguished Young Scholars(№30425010);National Natural Science Foundation of China(№30571971);the State Major Basic Key Research Program(№2006CB503807).

摘　　要：Aim： To investigate the interaction between hydrogen sulfide （H2S）/cystathionine γ-lyase （CSE） and carbon monoxide （CO）/heme oxygenase （HO） pathways in aortic smooth muscle cells （ASMC）. Methods： The ASMCs were divided into the following groups： （1） the control group; （2） the zinc protoporphyrin （ZnPP） 20 μmol/L group; （3） the propargylglycine （PPG） 2 mmol/L, 4 mmol/L and 10 mmol/L groups; and （4） the sodium hydrosulfide （NaHS） 1× 10^-5 mol/L, 1× 10^4 mol/L and 1× 10^-3 mol/L groups. Each of the groups was further divided into 6 h, 12 h, 18 h and 24 h subgroups. The CO level, represented by carboxyhemoglobin （HbCO） content was measured using a spectrophotometric method and HES content was detected by a sensitive electrode method. CSE and HO-1 expressions were detected by Western blotting. Results： The H2S content in the medium and CSE expression by ASMC were markedly increased by ZnPP compared with the control group. HbCO content in the medium and HO-1 expression by the ASMC started strengthening following 24 h treatment with PPG at 2 mmol/L, but were further strengthened following 18 h and 24 h treatment with PPG at 4 mmol/L compared with the controls （P〈0.01）. PPG at 10 mmol/L increased the HbCO level in the medium following 18 h treatment and increased HO-1 expression by the ASMC following 12 h treatment. Moreover, NaHS at 1× 10^-5 mol/L and 1× 10^-4 mol/L decreased the HbCO level in the medium and HO-1 expression by the ASMC after 6 h and 12 h treatment, while NaHS at 1× 10^-3 mol/L decreased them at all time points of the treatments. Conclusion： The results suggested that endogenous CO/HO and HES/CSE pathways inhibited each other in ASMC under physiological conditions.Aim： To investigate the interaction between hydrogen sulfide （H2S）/cystathionine γ-lyase （CSE） and carbon monoxide （CO）/heme oxygenase （HO） pathways in aortic smooth muscle cells （ASMC）. Methods： The ASMCs were divided into the following groups： （1） the control group; （2） the zinc protoporphyrin （ZnPP） 20 μmol/L group; （3） the propargylglycine （PPG） 2 mmol/L, 4 mmol/L and 10 mmol/L groups; and （4） the sodium hydrosulfide （NaHS） 1× 10^-5 mol/L, 1× 10^4 mol/L and 1× 10^-3 mol/L groups. Each of the groups was further divided into 6 h, 12 h, 18 h and 24 h subgroups. The CO level, represented by carboxyhemoglobin （HbCO） content was measured using a spectrophotometric method and HES content was detected by a sensitive electrode method. CSE and HO-1 expressions were detected by Western blotting. Results： The H2S content in the medium and CSE expression by ASMC were markedly increased by ZnPP compared with the control group. HbCO content in the medium and HO-1 expression by the ASMC started strengthening following 24 h treatment with PPG at 2 mmol/L, but were further strengthened following 18 h and 24 h treatment with PPG at 4 mmol/L compared with the controls （P〈0.01）. PPG at 10 mmol/L increased the HbCO level in the medium following 18 h treatment and increased HO-1 expression by the ASMC following 12 h treatment. Moreover, NaHS at 1× 10^-5 mol/L and 1× 10^-4 mol/L decreased the HbCO level in the medium and HO-1 expression by the ASMC after 6 h and 12 h treatment, while NaHS at 1× 10^-3 mol/L decreased them at all time points of the treatments. Conclusion： The results suggested that endogenous CO/HO and HES/CSE pathways inhibited each other in ASMC under physiological conditions.

关 键 词：hydrogen sulfide cystathionine γ-lyase carbon monoxide heme oxygenase 

分 类 号：R543.5[医药卫生—心血管疾病]