CCR3 monoclonal antibody inhibits airway eosinophilic inflammation and mucus overproduction in a mouse model of asthma  

作　　者：Hua-hao SHEN Feng XU Gen-sheng ZHANG Shao-bin WANG Wei-hua XU 

机构地区：[1]Department of Respiratory Diseases, the Second Affiliated Hospital, Zhejiang University College of Medicine, Hangzhou 310009, China

出　　处：《Acta Pharmacologica Sinica》2006年第12期1594-1599,共6页中国药理学报（英文版）

基　　金：Project supported in part by the National Natural Science Foundation of China(No 30570807);key programs of the Natural Science Foundation of Zhejiang Province,China(№Z303761);key programs of Science and Technology Association of Zhejiang Province(№2003C24002).

摘　　要：Aim： To explore the effect of a rat anti-mouse CC-chemokine receptor-3 （CCR3） monoclonal antibody （CCR3 mAb） on airway eosinophilia and mucus overproduction in asthmatic mice. Methods： An asthma model was sensitized and challenged by ovalbumin （OVA） in male C57BL/6 mice. Asthmatic mice were given dual administration （intraperitoneal injection and aerosol inhalation） of CCR3 mAb or nonspecific rat IgG （ns-IgG）. The number of total and differential inflammatory cells in the bronchial alveolar lavage fluid （BALF） was counted. Eosinophils number, the goblet cell percentage （GCP） and airway mucus index （AMI） were measured in the lung tissues. Interleukin （IL）-5 levels in the BALF were examined. The expression of MUC5AC and the epidermal growth factor receptor （EGFR） mRNA in the lung tissues was detected by semi-quantitative RT-PCR. The results were compared among the groups. Results： CCR3 mAb significantly suppressed the increased eosinophils in the BALF and lung tissues in OVA-challenged mice compared with ns-IgG-treated mice. IL-5 levels in the BALF in CCR3 mAb and ns-IgG administration mice exhibited no obvious changes relative to OVA-challenged asthmatic mice. CCR3 mAb reduced the increased GCP and AMI after OVA challenge and decreased the enhanced expression of MUC5AC and EGFR mRNA in lung tissues in asthmatic animals. Conclusion： CCR3 mAb can significantly inhibit airway eosinophilia and mucus overproduction in asthmatic mice. Blockage of CCR3 may represent a new strategy to asthma therapy.Aim： To explore the effect of a rat anti-mouse CC-chemokine receptor-3 （CCR3） monoclonal antibody （CCR3 mAb） on airway eosinophilia and mucus overproduction in asthmatic mice. Methods： An asthma model was sensitized and challenged by ovalbumin （OVA） in male C57BL/6 mice. Asthmatic mice were given dual administration （intraperitoneal injection and aerosol inhalation） of CCR3 mAb or nonspecific rat IgG （ns-IgG）. The number of total and differential inflammatory cells in the bronchial alveolar lavage fluid （BALF） was counted. Eosinophils number, the goblet cell percentage （GCP） and airway mucus index （AMI） were measured in the lung tissues. Interleukin （IL）-5 levels in the BALF were examined. The expression of MUC5AC and the epidermal growth factor receptor （EGFR） mRNA in the lung tissues was detected by semi-quantitative RT-PCR. The results were compared among the groups. Results： CCR3 mAb significantly suppressed the increased eosinophils in the BALF and lung tissues in OVA-challenged mice compared with ns-IgG-treated mice. IL-5 levels in the BALF in CCR3 mAb and ns-IgG administration mice exhibited no obvious changes relative to OVA-challenged asthmatic mice. CCR3 mAb reduced the increased GCP and AMI after OVA challenge and decreased the enhanced expression of MUC5AC and EGFR mRNA in lung tissues in asthmatic animals. Conclusion： CCR3 mAb can significantly inhibit airway eosinophilia and mucus overproduction in asthmatic mice. Blockage of CCR3 may represent a new strategy to asthma therapy.

关 键 词：ASTHMA CCR3 monoclonal antibody eosinophilic inflammation mucus overproduction MUC5AC epidermal growth factor receptor 

分 类 号：R441[医药卫生—诊断学]