与人PC-1同源的鼠PC-1基因的启动子的分子克隆和特性分析(英文)  

Molecular Cloning and Characterization of a Putative Promoter Region of mPC-1 Gene Homologous to hPC-1

作　　者：梁瑞霞[1] 涂智杰[2] 王健[1] 张惠[1] 姜飞[3] 庞博[1] 郑斌[1] 李素萍[2] 施庆国[1] 黄翠芬[1] 周建光[1] 

机构地区：[1]军事医学科学院生物工程研究所,北京100850 [2]厦门大学,福建厦门361005 [3]西北农林科技大学,陕西杨林712100

出　　处：《中国生物化学与分子生物学报》2006年第11期856-861,共6页Chinese Journal of Biochemistry and Molecular Biology

基　　金：国家高技术研究发展计划(863计划)(No.2002AA223061);国家自然科学基金(No.30070296)资助~~

摘　　要：为了鉴定鼠mPC-1基因表达的调控元件,克隆并分析了该基因的启动子.构建了一系列mPC-1基因启动子的截短序列.通过荧光素酶报道基因,分析了它们在前列腺癌细胞和其它细胞中的表达.结果表明,在AR阳性细胞系中,mPC-1基因启动子活性远远高于SV40和p61-PSA启动子,mPC-1基因启动子599bp至449bp可能含有一个负调控元件;mPC-11.1kb启动子控制的表达主要在前列腺癌细胞系中;雄激素可调控mPC-11.1kb启动子表达.mPC-11.1kb序列是一个有前列腺癌细胞特异性和较强的启动子,经过进一步的修饰有可能作为一种有用的前列腺癌基因治疗元件.To identify the regulatory region that are responsible for the expression of mPC-1, we have isolated and characterized the mPC-1 gene promoter. Sequence analysis of the mPC-1 5＇ -flanking region and a series of truncated constructs were performed, which were transiently transfected into the prostate cancer cell lines and non-prostate cancer cell lines and analyzed through Dual-luciferase reporter assay system. The relative activity of mPC-1 gene promoter was by far higher than pGL3-control containing SV40 promoter and enhancer and p61-PSA containing hPSA 6 kb promoter in AR （androgen receptor, AR）-positive prostate cancer cell lines. The region from 599 bp to 449 bp of mPC-1 promoter might contain a negative regulatory element. The expression of mPC-1 1.1 kb fragment is mainly restricted into prostate cancer cell lines. The relative activity of mPC-1 1.1 kb 5＇-flanking region was regulated by androgen. The results demonstrated that the 1.1 kb fragment of mPC-1 5＇ -flanking region was relatively strong and prostate cancer cell specific promoter region. The 1.1 kb promoter of mPC-1 gene might be well suited to prostate cancer gene therapy if the promoter was properly modified.

关 键 词：mPC-1基因 启动子 前列腺癌 LNCAP细胞 

分 类 号：Q785[生物学—分子生物学]