新疆军垦型细毛羊基因组BAC文库的构建  

Genome BAC Library Constrcution of Xinjiang Military Reclamation of Wasteland Type Fine-Fleece Sheep

作　　者：李谨[1] 杨公社[2] 陈创夫[1] 刘海波[3] 马润林[3] 王金富[1] 

机构地区：[1]石河子大学动物科技学院,新疆石河子832003 [2]西北农林科技大学动物科技学院,陕西杨凌712100 [3]中国科学院遗传与发育研究所,北京100101

出　　处：《中国生物化学与分子生物学报》2006年第11期880-885,共6页Chinese Journal of Biochemistry and Molecular Biology

基　　金：国家重大基础研究前期研究专项资助项目(No.2002CCC2100)~~

摘　　要：选用新疆特有的军垦型细毛羊为材料,构建了含有190464个克隆的全基因组BAC文库.文库的平均插入片段大小为133kb,同时文库92.5%克隆插入片段大于100kb,而且有部分克隆甚至大于300kb,这将满足大多数基因筛选的要求.假定绵羊的基因组含有3×106kb,根据文库的平均插入片段大小为133kb,计算的文库基因组覆盖率为8倍.因此,从文库筛选到目的片段的概率为98.208%.该文库中插入的外源片段来自新疆军垦型细毛羊的基因组,这对于研究新疆军垦型细毛羊的特殊性状的基因与其它绵羊品种和物种之间的差异,以及构建其全基因组物理图谱和基因图谱的完善是非常有利的.A BAC library containing 190 464 clones was constructed, in which the average insert DNA fragments was 133 kb, and 92.5% of fragments were longer than 100 kb, and some even longer than 300 kb. The insert fragments meet the needs for screening of most genes. If sheep＇s genome contains 3 × 10^6 kilobase, the library could cover 8 times of genome. The probability of screening the targat fragment from the library would be about 98 % . In this BAC library, the foreign fragment being inserted came from the Xinjiang military reclamation of wasteland type fine-fleece sheep＇s genome, which would be suitable for studying the specific genes of the sheep and the differences between the Xinjiang fine-fleece sheep and other species. This genome BAC library would be also convenient for genome physical mapping and improving the gene map of the Xinjiang fine-fleece sheep＇s .

关 键 词：新疆军垦型细毛羊 基因组 BAC文库 插入片段 覆盖率 

分 类 号：Q785[生物学—分子生物学]