一株急性散发性戊型肝炎病毒基因结构及核苷酸同源性分析  

Analysis of HEV genetic structure and nucleotide homology isolated from sporadic acute hepatitis E in Changchun

作　　者：刘子玲[1] 迟宝荣[2] 高桥和明 三代俊治 

机构地区：[1]吉林大学第一医院血液科,吉林长春130021 [2]吉林大学第一医院消化内科,吉林长春130021 [3]日本东芝病院肝病研究所

出　　处：《吉林大学学报（医学版）》2006年第6期1052-1054,共3页Journal of Jilin University：Medicine Edition

基　　金：国际合作项目(中国东北地区肝病疫学调研)资助课题(2005-2007年)

摘　　要：目的：分析一株长春地区散发戊型肝炎病毒（HEV）的基因结构和核苷酸同源性。方法：采用RT—nPCR方法检测HEVRNA，应用全自动测序仪进行序列测定。结果：所获得的HEV-CCC220全长7193bp，编码2397个氨基酸，由5＇-UTR（nt1-9）、3＇-UTR（nt 7152—7193）和3个开放读码框架（ORFs）组成。与HEVIV型核苷酸同源性明显高于HEVⅠ～Ⅲ型，全基因序列核苷酸同源性为83．2％～85．0％；与ORF2间300nt和98nt部分基因序列核苷酸同源性分别为85．0％～93．9％和80．6％～86．7％。结论：长春地区散发性HEV-CCC220属HEVIV型，ORF2间300nt部分核苷酸序列可以代替全基因序列进行HEV基因型分析。Objective To analyze the HEV genetic structure and nucleotide homology isolated from sporadic acute hepatitis E in Changchun. Methods HEV RNA was detected with reverse transcription nested polymerase （RT- nPCR） and sequenced by automatic sequenator. Results The length of acquisitive HEV-CCC220 was 7193 bp coding 2397 amino acid . The gene of HEV-CCC220 was composed of 5＇ -UTR （nt 1-＇9）, 3＇ - UTR （nt 7152- 7193） and three open reading frames （ORFs）. Its homology with HEV IV was obviously higher than that with HEV Ⅰ-Ⅲ. The whole genome sequence nucleotide homology was 83.2% -85.0%, 85.0% -93.9% and 80.6% -86.7% as compared with 300nt and 98nt partial nucleotide sequence of ORF2. The homology comparison of various areas and various lengths of nucleotide was different. Conclusion The HEV-ccc220, which sporadically happened in Changchun, is the subtype IV of HEV. The 300nt of ORF2 can replace the whole gene order to perform genotype analysis of HEV.

关 键 词：肝炎病毒 戊型 基因 结构 病毒 序列同源性 核酸 序列分析 

分 类 号：R373.2[医药卫生—病原生物学]