粉红粘帚霉67-1菌株原生质体的形成和再生  

Formation and Regeneration of Protoplasts in Gliocladium roseum 67-1

作　　者：张拥华[1] 李晶晶[2] 彭志刚[2] 李世东[1] 谢响明[2] 

机构地区：[1]中国农业科学院植物保护研究所,北京100081 [2]北京林业大学生物科学与技术学院,北京100083

出　　处：《中国生物防治》2006年第4期303-307,共5页Chinese Journal of Biological Control

基　　金：国家科技攻关计划"粮食作物重大病虫害防治技术研究"(2004BA520A15)

摘　　要：研究了多种细胞壁降解酶的种类和浓度、酶解时间、菌龄,以及渗透压稳定剂等因素对粉红粘帚霉67-1菌株原生质体形成和再生的影响。结果表明,粉红粘帚霉67-1菌株原生质体形成的适宜条件为:使用溶壁酶(3mg/ml)、蜗牛酶(3mg/ml)、纤维素酶(2mg/ml)的组合酶液酶解时间为2h,菌龄为14h,稳渗剂为NaCl(0·5mol/ml)。在上述条件下,原生质体产量达到9·25×106/ml。将原生质体涂布于含KCl(0·5mol/ml)的再生培养基上,再生率最高。显微观察表明,粉红粘帚霉67-1菌株主要通过顶端和侧位释放原生质体。The authors studied the effects of lytic enzymes combination, enzymatic time, mycelial age and osmotic stabilizer on the formation and regeneration of Gliocladium roseum 67-1 protoplasts, a highly potential biocontrol agent against plant fungal diseases. Results showed that the optimum conditions for the release of protoplast were enzyme combination of lywallzyme （3 mg/ml）, snailase （3 mg/ml） and cellulose （2 mg/ml） with 2 h of enzymatic time, mycelia aged 14 h and NaCl （0.5 mol/ml） as osmotic stabilizer. The optimum osmotic stabilizer for the regeneration of protoplast was KCl （0.5 mol/ml）. Microscopical observation indicated that the protoplasts of G. roseum 67-1 were largely released from the apex and the flank of hyphae.

关 键 词：粉红粘帚霉 原生质体 释放 再生 

分 类 号：S432.44[农业科学—植物病理学] S476[农业科学—农业昆虫与害虫防治]