维生素E对大鼠肝星状细胞增殖及细胞外基质的影响  

Inhibition roles of vitamin E on proliferation and secretion of extracellular matrix in rat hepatic stellate cells

作　　者：高润平[1] 杨秀云[1] 齐晓艳[1] 

机构地区：[1]吉林大学第一医院感染症科,吉林长春130021

出　　处：《中国老年学杂志》2006年第11期1503-1504,共2页Chinese Journal of Gerontology

基　　金：吉林省科技厅资助项目(1990316;200505211)

摘　　要：目的观察维生素E(VE)对大鼠肝星状细胞(HSC)过氧化脂质和细胞外基质(ECM)产生的影响。方法采用原位灌注方法分离大鼠HSC。结果新分离的大鼠HSC培养1w时具有增殖和ECM分泌的活性。VE可有效抑制大鼠HSC3H-胸腺嘧啶(3H-TdR)、3H-脯氨酸(3H-Pro)的掺入和Ⅲ型前胶原(PCⅢ)及透明质酸(HA)的分泌,VE作用后HSC产生丙二醛(MDA)下降,与分泌PCⅢ的抑制呈正相关。结论VE可抑制大鼠HSC增殖和减少ECM的分泌。Objective To study the inhibitory roles of vitamin E （VE） on proliferation and extracellular matrix （ECM） of hepatic stellate cells （HSCs） in vitro. Methods Rat HSCs were isolated and cultured in situ perfusion. Results The HSCs isolated and cultured by day 7 had abilities of proliferation and secretion of ECM and that VE had obvious effects in inhibiting the incorporation of ^3H-thymidine and ＆3H-proline and decreasing the secretion of precollagen type Ⅲ and hyaluronic acid, and that VE could markedly lower the production of malondialdehyde and inhibit the secretion of precollagen type Ⅲ with a positive correlation between them. Conclusions VE could inhibit the proliferation and secretion of ECM in rat HSC in vitro.

关 键 词：维生素E 肝星状细胞 细胞外基质 

分 类 号：R332[医药卫生—人体生理学]