金思维对脑缺血模型大鼠海马CRF,PKC蛋白表达的影响  

Influence of Chinese herbal extract complex on expression of Corticotropin-releasing factor and protein kinasec protein in hippocampus of middle cerebral artery occlusion rats

作　　者：尹军祥[1] 田金洲[1] 时晶[1] 程龙[1] 王永炎[2] 

机构地区：[1]北京中医药大学老年医学研究所,北京100700 [2]中国中医科学院,北京100700

出　　处：《中国中药杂志》2006年第23期1983-1986,共4页China Journal of Chinese Materia Medica

基　　金：国家重点基础研究发展(973)计划项目(2003CB517104);教育部重大科技项目(10408)

摘　　要：目的:观察中药金思维对脑缺血模型大鼠海马神经细胞促肾上腺皮质激素释放因子(CRF),蛋白激酶C(PKC)蛋白表达的影响。方法:采用线栓法制备脑缺血模型,大鼠随机分为假手术组,脑缺血模型组,金思维组(6.1 g.kg-1.d-1)和都可喜组(7.39 mg.kg-1.d-1)。利用免疫组化技术,分析各组大鼠海马区CRF,PKC蛋白表达量。结果:CRF:假手术组海马区可见少量阳性细胞;模型组见大量阳性细胞,着色深,随时间延长增多。金思维组和都可喜组阳性表达较少,着色较浅。模型组蛋白阳性表达面积均显著高于假手术组、金思维组和都可喜组,P<0.01。PKC:假手术组海马区阳性表达颗粒少,模型组见大量阳性表达颗粒,金思维组和都可喜组阳性表达颗粒稀疏。模型组CRF蛋白阳性表达面积均显著高于假手术组、金思维组和都可喜组,P<0.01。同时发现金思维组和都可喜组2组大鼠海马CRF,PKC蛋白阳性表达面积比较无明显差异。结论:脑缺血诱导CRF,PKC蛋白高表达是导致海马神经细胞迟发性死亡的一个重要因素;金思维可下调脑缺血后CRF,PKC蛋白表达,这可能是金思维保护缺血后神经损伤的作用机制之一。Objective： To explore the influence of one Chinese herbal extract complex （GETO） on the expression of corticotropin-releasing factor（CRF） and protein kinasec（PKC） proteins of the hlppocampus in middle cerebral artery orilusion（MCAO） rats. Method. All rats were subjected to MCAO by nylon thread, except the sham-group rats. Rats were divied into four groups：sham-group, cerebral ischemia model-group, GETO-group（6. 1 g·kg^-1·d^-1 ）and Duxil-group （7. 3 mg ·kg^-1·d^-1）. Using immunohistochemistry technique we measured the expression quantity of CRF and PKC protein in hippocampus of MCAO rats at 2 h,6 h and 24 h after reperfusion, contrasted to Duxil. Result： CRF： There were lots of positive and deeper dyeing neurons in hippocampus of model-group rats, while there were a few of positive and lighter dyeing neurons in sham-group, GETO-group and Duxil-group. The positive expression areas of CRF protein in hippocampus of model-group was significantly bigger than that of sham-group, GETO -group and Duxilgroup respectively（P 〈0. 01 ）. PKC：There were a great number of denser positive granules in hippocampus of model-group rats, while there were a few of scattered positive granules in sham-group, GETO-group and Duxil-group. The positive expression areas of CRF protein in hippocampus of model-group was significantly bigger than that of sham-group, GETO-group and Duxil-group respectively（P 〈 0. 01 ）. At the same time there was not significant difference about the expression of CRF and PKC protein between GETO group and Duxil-group. Conclusion： The high expression of CRF and PKC induced by cerehral ischemia may be one important factor that resulted in the delayed neuronal death in hippocampus. GETO can down-regulate the expression of CRF and PKC induced by cerebral iachemia, which may be one of the mechanisms that the Chinese herbal extract complex, protect cerebral ischemic injury.

关 键 词：金思维 脑缺血 促肾上腺皮质激素释放因子 蛋白激酶C 

分 类 号：R285.5[医药卫生—中药学]