绿僵菌防治椰心叶甲的毒力菌株筛选  

The virulence selection of Metarhizium spp.against the Brontispa longissium(Gastro)

作　　者：宋妍[1] 詹儒林[1] 张世清[1] 郭立佳[1] 许天委[1] 黄俊生[1] 

机构地区：[1]中国热带农业科学院环境与植物保护研究所热带作物生物技术国家重点实验室,海南海口571101

出　　处：《植物检疫》2006年第6期333-336,共4页Plant Quarantine

基　　金：国家科技部"科技基础性工作和社会公益研究专项"(编号:2004DIB3J073);科技部"国家科技基础条件平台工作"(编号:2004DKA30506)

摘　　要：在室内用绿僵菌的分生孢子悬浮液喷洒椰心叶甲成虫和幼虫,检测了不同来源的21株绿僵菌菌株对椰心叶甲的致病力。初步试验结果表明,A类菌株中的MA2、MA4和MA9,B类菌株中的MB2、MB4、MB5、MB6和MB7等对椰心叶甲的成虫和幼虫均具有较理想的效果,处理5天后成虫和幼虫的平均僵死率可达95%以上。但由于A类菌株比B类菌株产孢快、孢子量多,所以对A类菌株的MA2、MA4和MA9菌株再做进一步的室内毒力试验。最终结果表明,MA4菌株对椰心叶甲的毒力相对是最高的,具有较大的开发利用价值。Laboratory atomization of imagines and larvae of the Brontispa longissium （Gastro） with Metarhizium spp. spore suspension,Twenty-one strains of entomogenous fungus Metarhizium spp. were examined the pathogenicity with the Brontispa longissium （Gastro）. The first resuhs showed that MA2, MA4 and MA9 of the A type strains, MB2 ,MIM ,MB5 ,MB6 and MB7 of the B type strains were great pathogenicity to the Brontispa longissium（Gastro）. The average death rate of imagines and larvae of the Brontispa longissium（Gastro） were 95% after 5 days. But because the A type strains more fast and have more spores than the B type strains ,we did the further laboratory virulence test to MA2, MA4 and MA9. The last result showed, the virulence of MA4 was the most great having great value in controlling Brontispa longissium （Gastro）.

关 键 词：绿僵菌 椰心叶甲 毒力筛选 

分 类 号：S476.1[农业科学—农业昆虫与害虫防治]