抗毒死蜱单克隆抗体的研制  

Study on the monoclonal antibody to chlorpyrifos

作　　者：金仁耀[1] 桂文君[1] 吴建祥[2] 王春梅[1] 朱国念[1] 

机构地区：[1]浙江大学农药与环境毒理研究所,浙江杭州310029 [2]浙江大学生物技术研究所,浙江杭州310029

出　　处：《浙江大学学报（农业与生命科学版）》2006年第6期591-597,共7页Journal of Zhejiang University:Agriculture and Life Sciences

基　　金：国家自然科学基金资助项目(30370944)

摘　　要：用与牛血清蛋白(BSA)交联的毒死蜱人工抗原(CHBu-BSA)免疫的BALB/C鼠脾细胞与SP2/0鼠骨髓瘤细胞融合,经筛选和克隆,得到了1株能稳定分泌毒死蜱抗体的单克隆细胞株(1G10),1G10的抗体类型及亚类均为IgG1,其轻链为κ链.制备单克隆抗体腹水,腹水的间接ELISA效价在1×10-6以上.该单克隆抗体与毒死蜱特异性结合反应的50%抑制质量浓度为80.8μg?L-1,与其他毒死蜱结构类似物无交叉反应.One hybridoma cell line （1G10） secreting monoclonal antibody （McAb） against chlorpyrifos was produced by fusing mouse myeloma cells （SP2/0） with spleen cells from BALB/C which were immunized by the artificial antigen conjugated with bovine serum albumin （BSA）. Isotype and subclass of the monoclonal cell （1G10） was classified as IgG1. The light chain of the McAb was indentified to be κ. Its titres of ascitic fluids were up to 1 × 10^-6 by indirect ELISA. The inhibition rate of 50% of the McAb with the insecticide chlorpyrifos detected by ELISA was 80. 8 μg·L^-1, the McAb could specifically react with chlorpyrifos but no cross-reactivity to chlorpyrifos analogues.

关 键 词：毒死蜱 单克隆抗体 ELISA 

分 类 号：S482.3[农业科学—农药学]