酵母SAMDC基因表达载体构建以及农杆菌介导转化番茄的研究  

Construction of gene expression vector of yeast SAMDC and tomato transformation mediated by Agrobacterium tumefaciens

作　　者：丁淑丽[1] 李建勇[1] 邹宜静[1] 卢钢[1] 曹家树[1] 

机构地区：[1]浙江大学园艺系农业部园艺作物生长发育与生物技术重点实验室,浙江杭州310029

出　　处：《浙江大学学报（农业与生命科学版）》2006年第6期621-627,共7页Journal of Zhejiang University:Agriculture and Life Sciences

基　　金：国家自然科学基金资助项目(30571268);国家高技术研究发展计划863计划资助项目(2002AA207013)

摘　　要：SAMDC(腺苷甲硫氨酸脱羧酶)是参与植物多胺合成的一个关键酶.通过GenBank上已经发表的序列,设计两对引物,分别以酵母基因组DNA和番茄基因组DNA为模板克隆出SAMDC基因和E8启动子.构建了以CaMV35S和E8为启动子的两个SAMDC基因植物表达载体,酶切分子鉴定后,经农杆菌介导以叶盘法转入番茄中,获得了转基因植株.经PCR检测和X-Gluc组织化学染色检测,目的基因已经成功转入番茄中.为研究多胺在番茄中代谢活性以及SAMDC基因对番茄抗逆性和番茄果色的影响提供了研究基础.S-adenosylmethionine decarboxylase （SAMDC） is a key enzyme involved in the biosynthesis of the polyamines, viz. spermidine and spermine. Two pairs of primers were designed based on the published sequences in the GenBank. The SAMDC gene and E8 promoter were amplified using yeast and tomato genome DNA as templates, respectively. Then two SAMDC gene expression vectors containing CaMV35S and E8 promoters separately were constructed. The SAMDC gene was introduced into tomato genome by Agrobacteriurn tumefaciens mediated transformation, The PCR and X-Gluc histochemical assay results showed that SAMDC had been integrated into the genome of tomato successfully. The results provided a helpful tool for understanding the physiological function of polyamines and the effects of SAMDC gene on resistance to abiotic stress and fruit color in tomato.

关 键 词：SAMDC基因 E8启动子 番茄 载体构建 农杆菌介导 

分 类 号：S641.2[农业科学—蔬菜学] Q78[农业科学—园艺学]