纤维蛋白原Bβ链基因-1420G/A、-993C/T和-854G/A多态性与冠状动脉粥样硬化性心脏病的关联研究  

Association of fibrinogen B beta-1420G/A,-993C/T and-854G/A gene polymorphism with coronary heart diseas

作　　者：邢宏运[1] 蔡望伟[2] 李晓明[1] 符俏[3] 刘国勋[4] 

机构地区：[1]四川省泸州医学院附属医院血液科,646000 [2]海南医学院生物化学教研室 [3]海南省人民医院神经内科 [4]广东医学院附属医院血液研究室

出　　处：《中华医学遗传学杂志》2006年第6期622-626,共5页Chinese Journal of Medical Genetics

基　　金：国家自然科学基金(30060037);教育部科学技术研究重点项目(03147);广东省卫生厅科研项目(B2000121)~~

摘　　要：目的调查健康人和冠状动脉粥样硬化性心脏病(简称冠心病)患者的纤维蛋白原B(fibrino-genB,FGB)β基因-1420G/A、-993C/T和-854G/A的基因多态性频率分布,以及与血浆Fg水平的关系。方法应用等位基因特异性PCR扩增技术和限制性片段长度多态性技术对186例冠心病患者和149名健康对照者进行分析。比浊法测定血浆Fg浓度。结果等位基因频率-1420A在冠心病组为0.33,在对照组为0.26,冠心病组明显比对照组升高,两者比较差异有统计学意义(P<0.05)。等位基因频率-993T和-854A在两组之间无差别。Logistic回归分析显示β-1420G/A与冠心病相关(OR=1.922,P=0.003)。冠心病组血浆Fg水平(3.87±1.75)g/L较健康人组(3.10±0.77)g/L明显升高,且差异有统计学意义(P<0·05)。结论纤维蛋白原Bβ-1420G/A可能与冠心病发病相关联。Objective To analyze the frequency of FGB gene -1420G/A, -993C/T and -854G/A polymorphisms, and their association with plasma fibrinogen levels in patients with coronary heart disease and in health adults. Methods The FGB gene -1420G/A, -993C/T and -854G/A polymorphisms were analyzed with restriction fragment length polymorphisms, polymerase chain reaction with allele-specific primer and nucleotide sequencing methods. Plamsa fibrinogen levels were determined by turbidimetry. Results The frequencies of -1420A were 0.33 in patients with coronary heart disease and 0.26 in health adults. The frequencies of -1420A in coronary heart disease were apparently higher than that in health adults. There were no difference in frequencies of other two alleles. The logistic study suggested -1420G/A polymorphism was associated with coronary heart disease. There are significantly difference in plasma fibrinogen levels in two groups. Plasma fibrinogen levels were significantly increased in patients with coronary heart disease. Conclusion This study suggests -1420G/A polymorphism may be associated with occurrence of coronary heart disease.

关 键 词：纤维蛋白原 遗传多态性 冠状动脉粥样硬化性心脏病 

分 类 号：R541.4[医药卫生—心血管疾病]