Galβ-1,4-GlcNAc在钳夹伤后大鼠坐骨神经中的表达变化  

Expressive changes of Galβ-1,4-GlcNAc in the rat sciatic nerve after crushed

作　　者：朱敏[1] 沈爱国[2] 丁炯[1] 顾晓松[2] 王鹤鸣[1] 

机构地区：[1]南京医科大学人体解剖学系,南京210029 [2]南通大学神经再生重点实验室,南通226001

出　　处：《中国临床解剖学杂志》2006年第6期658-661,共4页Chinese Journal of Clinical Anatomy

基　　金：国家自然科学基金(30300099);江苏省自然科学基金(BK2003035);江苏省教育厅自然科学项目(03KJB180109);南京医科大学校科技发展基金(Ny04002)

摘　　要：目的:分析Galβ-1,4-GlcNAc在正常和钳夹伤后大鼠坐骨神经中的表达变化。方法:采用免疫荧光和凝集素组织化学的方法检测Galβ-1,4-GlcNAc在正常和夹伤的大鼠坐骨神经中的表达定位。然后用图像分析的方法分析Galβ-1,4-GlcNAc在正常和夹伤的大鼠坐骨神经中的表达变化。结果:运用免疫荧光、凝集素组织化学方法和图像分析的方法检测Galβ-1,4-GlcNAc在夹伤后的坐骨神经中的表达定位及变化,发现其表达在外周神经中的雪旺细胞中并于夹伤后的一周达到高峰,于夹伤后两周恢复至正常水平。结论:提示Galβ-1,4-GlcNAc在坐骨神经夹伤后发生表达变化,并且主要表达在坐骨神经的雪旺细胞中,说明Galβ-1,4-GlcNAc在周围神经再生的早期发挥着重要的作用。Objective： To investigate expressive changes of Ga1β-1, 4-G1cNAc and explore its potential roles for nerve regeneration after crushing rat sciatic nerve. Methods： The synthesis and localization of Ga1β-1, 4-G1cNAc in crushed sciatic nerves were studied by immunoftuorescence and lectin histochemistry of Ricinus Communis Agglutinin-Ⅰ （RCA-Ⅰ）. The expressive changes of them in normal and crushed sciatic nerves were analyzed and compared by image analysis. Results： The results indicated that the Ga1β-1, 4-G1cNAc localized mainly in Schwann cells of crushed nerves and reached the peak at the 7th day after the nerve crushed and decreased gradually. Conclusions： Ga1β-1, 4-G1cNAc appeared in Schwann cells of damaged nerves and might play a role in the nerve regeneration at the early stage after the sciatic nerve crushed.

关 键 词：Ga1β-1 4-G1cNAc 免疫荧光组织化学方法 凝集素组织化学方法 坐骨神经 大鼠 

分 类 号：R651.3[医药卫生—外科学]